Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84350
Title:
Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.
Authors:
Potten, Christopher S; Booth, Dawn; Cragg, N J; O'Shea, Julie A; Tudor, Gregory L; Booth, Catherine
Abstract:
Keratinocyte growth factor (KGF) stimulates proliferation and differentiation in various epithelial systems. Three daily subcutaneous injections of 125 microg of this protein into mice induce dramatic changes in the histology and histometric measurements of the ventral tongue epithelium. The thickness of the epithelium is increased two-fold and the number of cells beneath a 1-mm length of the surface is increased 1.6-fold. KGF also induces a four-fold increase in the number of S phase cells labelled with tritiated thymidine in the basal layer on the third day after KGF administration. The increase in thickness and cellularity persist for at least 4 days after the end of the KGF injections. However, there is a dramatic fall in the number of S phase cells detected by 3HTdR pulse labelling 2 days after the end of the KGF treatment. There are indications that by 7 days after the 3-day regimen of KGF treatment, both thickness and cellularity have fallen back to near control levels. Continued exposure to KGF over a period of 7 days does not result in any further increases in thickness, cellularity or proliferation. In fact, the proliferation decreases on the fifth, sixth and seventh days of KGF injection to control values on day 7. These changes in the epithelium following KGF treatment suggest that the thicker and more cellular epithelium may be more able to cope with an exposure to a cytotoxic agent and hence be protected in comparison with normal or vehicle-treated epithelium.
Affiliation:
Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.
Citation:
Cell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor. 2002, 35 Suppl 1:22-31 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
Aug-2002
URI:
http://hdl.handle.net/10541/84350
DOI:
10.1046/j.1365-2184.35.s1.3.x
PubMed ID:
12139705
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorBooth, Dawnen
dc.contributor.authorCragg, N Jen
dc.contributor.authorO'Shea, Julie Aen
dc.contributor.authorTudor, Gregory Len
dc.contributor.authorBooth, Catherineen
dc.date.accessioned2009-10-16T14:54:06Z-
dc.date.available2009-10-16T14:54:06Z-
dc.date.issued2002-08-
dc.identifier.citationCell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor. 2002, 35 Suppl 1:22-31 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid12139705-
dc.identifier.doi10.1046/j.1365-2184.35.s1.3.x-
dc.identifier.urihttp://hdl.handle.net/10541/84350-
dc.description.abstractKeratinocyte growth factor (KGF) stimulates proliferation and differentiation in various epithelial systems. Three daily subcutaneous injections of 125 microg of this protein into mice induce dramatic changes in the histology and histometric measurements of the ventral tongue epithelium. The thickness of the epithelium is increased two-fold and the number of cells beneath a 1-mm length of the surface is increased 1.6-fold. KGF also induces a four-fold increase in the number of S phase cells labelled with tritiated thymidine in the basal layer on the third day after KGF administration. The increase in thickness and cellularity persist for at least 4 days after the end of the KGF injections. However, there is a dramatic fall in the number of S phase cells detected by 3HTdR pulse labelling 2 days after the end of the KGF treatment. There are indications that by 7 days after the 3-day regimen of KGF treatment, both thickness and cellularity have fallen back to near control levels. Continued exposure to KGF over a period of 7 days does not result in any further increases in thickness, cellularity or proliferation. In fact, the proliferation decreases on the fifth, sixth and seventh days of KGF injection to control values on day 7. These changes in the epithelium following KGF treatment suggest that the thicker and more cellular epithelium may be more able to cope with an exposure to a cytotoxic agent and hence be protected in comparison with normal or vehicle-treated epithelium.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Division-
dc.subject.meshEpithelial Cells-
dc.subject.meshFibroblast Growth Factor 7-
dc.subject.meshFibroblast Growth Factors-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshThymidine-
dc.subject.meshTongue-
dc.subject.meshTritium-
dc.titleCell kinetic studies in the murine ventral tongue epithelium: the effects of repeated exposure to keratinocyte growth factor.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.en
dc.identifier.journalCell Proliferationen

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