Cell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84323
Title:
Cell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination.
Authors:
Potten, Christopher S; Booth, Dawn; Cragg, N J; Tudor, Gregory L; O'Shea, Julie A; Appleton, D R; Barthel, D; Gerike, T G; Meineke, F A; Loeffler, M; Booth, Catherine
Abstract:
The oral mucosa is a rapidly replacing body tissue that has received relatively little attention in terms of defining its cell kinetics and cellular organization. The tissue is sensitive to the effects of cytotoxic agents, the consequence of which can be stem cell death with the subsequent development of ulcers and the symptoms of oral mucositis. There is considerable interest in designing strategies to protect oral stem cells and, hence, reduce the mucositis side-effects in cancer therapy patients. Here we present details of a new histometric approach designed to investigate the changing patterns in cellularity in the ventral tongue mucosa. This initial paper in a series of four papers presents observations on the changing patterns in the labelling index following tritiated thymidine administration, which suggest a delayed uptake of tritiated thymidine from a long-term intracellular thymidine pool, a phenomenon that will complicate cell kinetic interpretations in a variety of experimental situations. We also provide data on the changing pattern of mitotic activity through a 24-h period (circadian rhythms). Using vincristine-induced stathmokinesis, the data indicate that 54% of the basal cells divide each day and that there is a high degree of synchrony in mitotic activity with a mitotic peak occurring around 13.00 h. The mitotic circadian peak occurs 9-12 h after the circadian peak in DNA synthesis. The data presented here and in the subsequent papers could be interpreted to indicate that basal cells of BDF1 mice have an average turnover time of about 26-44 h with some cells cycling once a day and others with a 2- or 3-day cell cycle time.
Affiliation:
Paterson Institute for cancer Research, Christie Hospital NHS Trust, wilmslow Road, Manchester M20 4BX, UK.
Citation:
Cell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination. 2002, 35 Suppl 1:1-15 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
Aug-2002
URI:
http://hdl.handle.net/10541/84323
DOI:
10.1046/j.1365-2184.35.s1.1.x
PubMed ID:
12139703
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorBooth, Dawnen
dc.contributor.authorCragg, N Jen
dc.contributor.authorTudor, Gregory Len
dc.contributor.authorO'Shea, Julie Aen
dc.contributor.authorAppleton, D Ren
dc.contributor.authorBarthel, Den
dc.contributor.authorGerike, T Gen
dc.contributor.authorMeineke, F Aen
dc.contributor.authorLoeffler, Men
dc.contributor.authorBooth, Catherineen
dc.date.accessioned2009-10-16T11:37:20Z-
dc.date.available2009-10-16T11:37:20Z-
dc.date.issued2002-08-
dc.identifier.citationCell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination. 2002, 35 Suppl 1:1-15 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid12139703-
dc.identifier.doi10.1046/j.1365-2184.35.s1.1.x-
dc.identifier.urihttp://hdl.handle.net/10541/84323-
dc.description.abstractThe oral mucosa is a rapidly replacing body tissue that has received relatively little attention in terms of defining its cell kinetics and cellular organization. The tissue is sensitive to the effects of cytotoxic agents, the consequence of which can be stem cell death with the subsequent development of ulcers and the symptoms of oral mucositis. There is considerable interest in designing strategies to protect oral stem cells and, hence, reduce the mucositis side-effects in cancer therapy patients. Here we present details of a new histometric approach designed to investigate the changing patterns in cellularity in the ventral tongue mucosa. This initial paper in a series of four papers presents observations on the changing patterns in the labelling index following tritiated thymidine administration, which suggest a delayed uptake of tritiated thymidine from a long-term intracellular thymidine pool, a phenomenon that will complicate cell kinetic interpretations in a variety of experimental situations. We also provide data on the changing pattern of mitotic activity through a 24-h period (circadian rhythms). Using vincristine-induced stathmokinesis, the data indicate that 54% of the basal cells divide each day and that there is a high degree of synchrony in mitotic activity with a mitotic peak occurring around 13.00 h. The mitotic circadian peak occurs 9-12 h after the circadian peak in DNA synthesis. The data presented here and in the subsequent papers could be interpreted to indicate that basal cells of BDF1 mice have an average turnover time of about 26-44 h with some cells cycling once a day and others with a 2- or 3-day cell cycle time.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntineoplastic Agents, Phytogenic-
dc.subject.meshCell Division-
dc.subject.meshCircadian Rhythm-
dc.subject.meshEpithelial Cells-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMitosis-
dc.subject.meshThymidine-
dc.subject.meshTongue-
dc.subject.meshTritium-
dc.subject.meshVincristine-
dc.titleCell kinetic studies in the murine ventral tongue epithelium: thymidine metabolism studies and circadian rhythm determination.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for cancer Research, Christie Hospital NHS Trust, wilmslow Road, Manchester M20 4BX, UK.en
dc.identifier.journalCell Proliferationen
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