Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84321
Title:
Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.
Authors:
Quinn, Janet; Findlay, Victoria J; Dawson, Keren; Millar, Jonathan B A; Jones, Nic; Morgan, Brian A; Toone, W Mark
Abstract:
The signaling pathways that sense adverse stimuli and communicate with the nucleus to initiate appropriate changes in gene expression are central to the cellular stress response. Herein, we have characterized the role of the Sty1 (Spc1) stress-activated mitogen-activated protein kinase pathway, and the Pap1 and Atf1 transcription factors, in regulating the response to H(2)O(2) in the fission yeast Schizosaccharomyces pombe. We find that H(2)O(2) activates the Sty1 pathway in a dose-dependent manner via at least two sensing mechanisms. At relatively low levels of H(2)O(2), a two component-signaling pathway, which feeds into either of the two stress-activated mitogen-activated protein kinase kinase kinases Wak1 or Win1, regulates Sty1 phosphorylation. In contrast, at high levels of H(2)O(2), Sty1 activation is controlled predominantly by a two-component independent mechanism and requires the function of both Wak1 and Win1. Individual transcription factors were also found to function within a limited range of H(2)O(2) concentrations. Pap1 activates target genes primarily in response to low levels of H(2)O(2), whereas Atf1 primarily controls the transcriptional response to high concentrations of H(2)O(2). Our results demonstrate that S. pombe uses a combination of stress-responsive regulatory proteins to gauge and effect the appropriate transcriptional response to increasing concentrations of H(2)O(2).
Affiliation:
Cancer Research UK Cell Regulation Group, Paterson Institute for Cancer Research. Christie Hospital, Withington, Manchester M20 4BX, UK
Citation:
Distinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe. 2002, 13 (3):805-16 Mol. Biol. Cell
Journal:
Molecular Biology of the Cell
Issue Date:
Mar-2002
URI:
http://hdl.handle.net/10541/84321
DOI:
10.1091/mbc.01-06-0288
PubMed ID:
11907263
Type:
Article
Language:
en
ISSN:
1059-1524
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorQuinn, Janeten
dc.contributor.authorFindlay, Victoria Jen
dc.contributor.authorDawson, Kerenen
dc.contributor.authorMillar, Jonathan B Aen
dc.contributor.authorJones, Nicen
dc.contributor.authorMorgan, Brian Aen
dc.contributor.authorToone, W Marken
dc.date.accessioned2009-10-16T11:19:28Z-
dc.date.available2009-10-16T11:19:28Z-
dc.date.issued2002-03-
dc.identifier.citationDistinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe. 2002, 13 (3):805-16 Mol. Biol. Cellen
dc.identifier.issn1059-1524-
dc.identifier.pmid11907263-
dc.identifier.doi10.1091/mbc.01-06-0288-
dc.identifier.urihttp://hdl.handle.net/10541/84321-
dc.description.abstractThe signaling pathways that sense adverse stimuli and communicate with the nucleus to initiate appropriate changes in gene expression are central to the cellular stress response. Herein, we have characterized the role of the Sty1 (Spc1) stress-activated mitogen-activated protein kinase pathway, and the Pap1 and Atf1 transcription factors, in regulating the response to H(2)O(2) in the fission yeast Schizosaccharomyces pombe. We find that H(2)O(2) activates the Sty1 pathway in a dose-dependent manner via at least two sensing mechanisms. At relatively low levels of H(2)O(2), a two component-signaling pathway, which feeds into either of the two stress-activated mitogen-activated protein kinase kinase kinases Wak1 or Win1, regulates Sty1 phosphorylation. In contrast, at high levels of H(2)O(2), Sty1 activation is controlled predominantly by a two-component independent mechanism and requires the function of both Wak1 and Win1. Individual transcription factors were also found to function within a limited range of H(2)O(2) concentrations. Pap1 activates target genes primarily in response to low levels of H(2)O(2), whereas Atf1 primarily controls the transcriptional response to high concentrations of H(2)O(2). Our results demonstrate that S. pombe uses a combination of stress-responsive regulatory proteins to gauge and effect the appropriate transcriptional response to increasing concentrations of H(2)O(2).en
dc.language.isoenen
dc.subject.meshActivating Transcription Factor 1-
dc.subject.meshBasic-Leucine Zipper Transcription Factors-
dc.subject.meshCell Nucleus-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshEnzyme Activation-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshHydrogen Peroxide-
dc.subject.meshMAP Kinase Kinase Kinases-
dc.subject.meshMAP Kinase Signaling System-
dc.subject.meshMitogen-Activated Protein Kinases-
dc.subject.meshModels, Biological-
dc.subject.meshOxidants-
dc.subject.meshOxidative Stress-
dc.subject.meshPeroxidases-
dc.subject.meshPhosphorylation-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.subject.meshTranscription Factors-
dc.subject.meshTranscription, Genetic-
dc.titleDistinct regulatory proteins control the graded transcriptional response to increasing H(2)O(2) levels in fission yeast Schizosaccharomyces pombe.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Cell Regulation Group, Paterson Institute for Cancer Research. Christie Hospital, Withington, Manchester M20 4BX, UKen
dc.identifier.journalMolecular Biology of the Cellen

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