Cord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84158
Title:
Cord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells.
Authors:
Summers, Yvonne J; Heyworth, Clare M; De Wynter, Erika A; Chang, James; Testa, Nydia G
Abstract:
We examined the functional differences between G(0) and G(1) cord blood CD34+ cells for up to 24 weeks in serum-free suspension culture, containing Flt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x 10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34+, resulting in a 600-fold expansion of CD34+ cells at week 8. As in other in vitro systems, total cell expansion is less discriminatory of stem cell behavior than progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks.
Affiliation:
CRC Experimental Haematology Group, Paterson Institute for Cancer Research, Manchester MZ0 4BX, United Kingdom. ysummers@picr.man.ac.uk
Citation:
Cord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells. 2001, 19 (6):505-13 Stem Cells
Journal:
Stem Cells
Issue Date:
2001
URI:
http://hdl.handle.net/10541/84158
DOI:
10.1634/stemcells.19-6-505
PubMed ID:
11713342
Type:
Article
Language:
en
ISSN:
1066-5099
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSummers, Yvonne Jen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorDe Wynter, Erika Aen
dc.contributor.authorChang, Jamesen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2009-10-13T10:38:44Z-
dc.date.available2009-10-13T10:38:44Z-
dc.date.issued2001-
dc.identifier.citationCord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells. 2001, 19 (6):505-13 Stem Cellsen
dc.identifier.issn1066-5099-
dc.identifier.pmid11713342-
dc.identifier.doi10.1634/stemcells.19-6-505-
dc.identifier.urihttp://hdl.handle.net/10541/84158-
dc.description.abstractWe examined the functional differences between G(0) and G(1) cord blood CD34+ cells for up to 24 weeks in serum-free suspension culture, containing Flt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x 10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34+, resulting in a 600-fold expansion of CD34+ cells at week 8. As in other in vitro systems, total cell expansion is less discriminatory of stem cell behavior than progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34-
dc.subject.meshCell Count-
dc.subject.meshCell Cycle-
dc.subject.meshCell Division-
dc.subject.meshFetal Blood-
dc.subject.meshFlow Cytometry-
dc.subject.meshG0 Phase-
dc.subject.meshG1 Phase-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshTime Factors-
dc.titleCord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells.en
dc.typeArticleen
dc.contributor.departmentCRC Experimental Haematology Group, Paterson Institute for Cancer Research, Manchester MZ0 4BX, United Kingdom. ysummers@picr.man.ac.uken
dc.identifier.journalStem Cellsen

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