Screening for herpesvirus genomes in common acute lymphoblastic leukemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84080
Title:
Screening for herpesvirus genomes in common acute lymphoblastic leukemia.
Authors:
MacKenzie, Jane; Gallagher, A; Clayton, R A; Perry, Jacqueline; Eden, Tim O B; Ford, A M; Greaves, M F; Jarrett, Ruth F
Abstract:
There is epidemiological evidence that infection may play a role in the etiology of childhood leukemia in particular common B cell precursor acute lymphoblastic leukemia. A panel of 20 leukemic samples (panel 1) was examined for the presence of four lymphotropic herpesviruses using conventional molecular techniques. A second independent panel of 27 leukemic samples (panel 2), along with 28 control peripheral blood samples from children with other forms of cancer, was tested for the presence of the same four viruses using sensitive real-time quantitative PCR. While herpesvirus genomes were detected, they were present at very low levels; detection rates and levels were similar in the leukemic and control panels. In addition we surveyed 18 leukemic samples (five from panel 1, six from panel 2 and a further seven samples not previously analyzed) using a degenerate PCR assay capable of detecting the genomes of known herpesviruses plus putative new members of the family. No novel herpesvirus genomes were detected suggesting that a herpesvirus is unlikely to be etiologically involved as a transforming agent in common acute lymphoblastic leukemia.
Affiliation:
LRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, UK.
Citation:
Screening for herpesvirus genomes in common acute lymphoblastic leukemia. 2001, 15 (3):415-21 Leukemia
Journal:
Leukemia
Issue Date:
Mar-2001
URI:
http://hdl.handle.net/10541/84080
PubMed ID:
11237065
Type:
Article
Language:
en
ISSN:
0887-6924
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorMacKenzie, Janeen
dc.contributor.authorGallagher, Aen
dc.contributor.authorClayton, R Aen
dc.contributor.authorPerry, Jacquelineen
dc.contributor.authorEden, Tim O Ben
dc.contributor.authorFord, A Men
dc.contributor.authorGreaves, M Fen
dc.contributor.authorJarrett, Ruth Fen
dc.date.accessioned2009-10-12T16:02:03Z-
dc.date.available2009-10-12T16:02:03Z-
dc.date.issued2001-03-
dc.identifier.citationScreening for herpesvirus genomes in common acute lymphoblastic leukemia. 2001, 15 (3):415-21 Leukemiaen
dc.identifier.issn0887-6924-
dc.identifier.pmid11237065-
dc.identifier.urihttp://hdl.handle.net/10541/84080-
dc.description.abstractThere is epidemiological evidence that infection may play a role in the etiology of childhood leukemia in particular common B cell precursor acute lymphoblastic leukemia. A panel of 20 leukemic samples (panel 1) was examined for the presence of four lymphotropic herpesviruses using conventional molecular techniques. A second independent panel of 27 leukemic samples (panel 2), along with 28 control peripheral blood samples from children with other forms of cancer, was tested for the presence of the same four viruses using sensitive real-time quantitative PCR. While herpesvirus genomes were detected, they were present at very low levels; detection rates and levels were similar in the leukemic and control panels. In addition we surveyed 18 leukemic samples (five from panel 1, six from panel 2 and a further seven samples not previously analyzed) using a degenerate PCR assay capable of detecting the genomes of known herpesviruses plus putative new members of the family. No novel herpesvirus genomes were detected suggesting that a herpesvirus is unlikely to be etiologically involved as a transforming agent in common acute lymphoblastic leukemia.en
dc.language.isoenen
dc.subjectPrecursor Cell Lymphoblastic Leukaemia-Lymphomaen
dc.subject.meshAdolescent-
dc.subject.meshBlotting, Southern-
dc.subject.meshChild-
dc.subject.meshChild, Preschool-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA Primers-
dc.subject.meshGenome, Viral-
dc.subject.meshHerpesviridae-
dc.subject.meshHumans-
dc.subject.meshInfant-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma-
dc.titleScreening for herpesvirus genomes in common acute lymphoblastic leukemia.en
dc.typeArticleen
dc.contributor.departmentLRF Virus Centre, Department of Veterinary Pathology, University of Glasgow, UK.en
dc.identifier.journalLeukemiaen

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