The human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/84047
Title:
The human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells.
Authors:
Ward, Christopher M; Stern, Peter L
Abstract:
It has been reported recently that the cytomegalovirus (CMV) immediate-early promoter is transcriptionally inactive in undifferentiated mouse embryonic stem (ES) cells. This result is surprising, since the CMV promoter is used to express transgenes in a variety of cell lines. We studied the expression of a human CMV-driven enhanced green fluorescent protein (EGFP) reporter gene (pEGFP-N1) in five undifferentiated mouse ES cell lines (BL/6III, D3, E14TG2a, MESC20, and 129) and found EGFP expression in all of these cell lines. Under optimal conditions, between 50%-80% transfection efficiencies could be achieved, and EGFP expression levels were maintained for at least 72 hours. Therefore, the human CMV promoter remains a useful system for transgene expression in undifferentiated ES cells.
Affiliation:
Immunology Group, Paterson Institute for Cancer Research (PICR), Christie Hospital NHS Trust, Manchester, United Kingdom. wardcm@hotmail.com
Citation:
The human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells. 2002, 20 (5):472-5 Stem Cells
Journal:
Stem Cells
Issue Date:
2002
URI:
http://hdl.handle.net/10541/84047
DOI:
10.1634/stemcells.20-5-472
PubMed ID:
12351818
Type:
Article
Language:
en
ISSN:
1066-5099
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWard, Christopher Men
dc.contributor.authorStern, Peter Len
dc.date.accessioned2009-10-12T12:24:09Z-
dc.date.available2009-10-12T12:24:09Z-
dc.date.issued2002-
dc.identifier.citationThe human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells. 2002, 20 (5):472-5 Stem Cellsen
dc.identifier.issn1066-5099-
dc.identifier.pmid12351818-
dc.identifier.doi10.1634/stemcells.20-5-472-
dc.identifier.urihttp://hdl.handle.net/10541/84047-
dc.description.abstractIt has been reported recently that the cytomegalovirus (CMV) immediate-early promoter is transcriptionally inactive in undifferentiated mouse embryonic stem (ES) cells. This result is surprising, since the CMV promoter is used to express transgenes in a variety of cell lines. We studied the expression of a human CMV-driven enhanced green fluorescent protein (EGFP) reporter gene (pEGFP-N1) in five undifferentiated mouse ES cell lines (BL/6III, D3, E14TG2a, MESC20, and 129) and found EGFP expression in all of these cell lines. Under optimal conditions, between 50%-80% transfection efficiencies could be achieved, and EGFP expression levels were maintained for at least 72 hours. Therefore, the human CMV promoter remains a useful system for transgene expression in undifferentiated ES cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCells, Cultured-
dc.subject.meshCytomegalovirus-
dc.subject.meshEmbryo, Mammalian-
dc.subject.meshFetus-
dc.subject.meshGene Expression Regulation, Viral-
dc.subject.meshGenes, Immediate-Early-
dc.subject.meshGenes, Reporter-
dc.subject.meshGreen Fluorescent Proteins-
dc.subject.meshHumans-
dc.subject.meshLuminescent Proteins-
dc.subject.meshMice-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshTotipotent Stem Cells-
dc.subject.meshTranscription, Genetic-
dc.subject.meshTransgenes-
dc.titleThe human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells.en
dc.typeArticleen
dc.contributor.departmentImmunology Group, Paterson Institute for Cancer Research (PICR), Christie Hospital NHS Trust, Manchester, United Kingdom. wardcm@hotmail.comen
dc.identifier.journalStem Cellsen

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