2.50
Hdl Handle:
http://hdl.handle.net/10541/83572
Title:
Targeted cytokine delivery to neuroblastoma.
Authors:
Dehal, P K; Embleton, Jim; Kemshead, John T; Hawkins, Robert E
Abstract:
The aim of this study was to construct a fusion protein from the cytokine granulocyte/macrophage colony-stimulating factor (GM-CSF) and a single-chain Fv fragment (scFv D29) and to investigate its potential to activate cells of the immune system against neuroblastoma cells expressing neural cell adhesion molecule (NCAM). Mammalian cell expression of the scFv D29-GM-CSF fusion protein was compared using a number of vectors, including retroviral and adenoviral vectors. The resultant fusion protein, expressed by HeLa cells, was found by ELISA to bind immobilized recombinant NCAM. Moreover, FACS analysis confirmed binding to the human neuroblastoma cell line SKNBE and a murine neuroblastoma cell line engineered to express the glycosylphosphatidylinositol form of human NCAM (N2A-rKNIE). The fusion protein was also found to stimulate the proliferation of the FDC-P1 haemopoietic cell line, which is dependent on GM-CSF (or interleukin 3) for continued growth. In vitro clonogenic assays indicated that scFv-GM-CSF could selectively induce growth inhibition of SKNBE cells by murine lymphoid cells.
Affiliation:
Medical Oncology, Paterson Institute for Cancer Research, Wilmslow Road, Withington, Manchester M20 4BX, UK.
Citation:
Targeted cytokine delivery to neuroblastoma. 2002, 30 (4):518-20 Biochem. Soc. Trans.
Journal:
Biochemical Society Transactions
Issue Date:
Aug-2002
URI:
http://hdl.handle.net/10541/83572
DOI:
10.1042/
PubMed ID:
12196127
Type:
Article
Language:
en
ISSN:
0300-5127
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDehal, P K-
dc.contributor.authorEmbleton, Jim-
dc.contributor.authorKemshead, John T-
dc.contributor.authorHawkins, Robert E-
dc.date.accessioned2009-10-05T15:16:38Z-
dc.date.available2009-10-05T15:16:38Z-
dc.date.issued2002-08-
dc.identifier.citationTargeted cytokine delivery to neuroblastoma. 2002, 30 (4):518-20 Biochem. Soc. Trans.en
dc.identifier.issn0300-5127-
dc.identifier.pmid12196127-
dc.identifier.doi10.1042/-
dc.identifier.urihttp://hdl.handle.net/10541/83572-
dc.description.abstractThe aim of this study was to construct a fusion protein from the cytokine granulocyte/macrophage colony-stimulating factor (GM-CSF) and a single-chain Fv fragment (scFv D29) and to investigate its potential to activate cells of the immune system against neuroblastoma cells expressing neural cell adhesion molecule (NCAM). Mammalian cell expression of the scFv D29-GM-CSF fusion protein was compared using a number of vectors, including retroviral and adenoviral vectors. The resultant fusion protein, expressed by HeLa cells, was found by ELISA to bind immobilized recombinant NCAM. Moreover, FACS analysis confirmed binding to the human neuroblastoma cell line SKNBE and a murine neuroblastoma cell line engineered to express the glycosylphosphatidylinositol form of human NCAM (N2A-rKNIE). The fusion protein was also found to stimulate the proliferation of the FDC-P1 haemopoietic cell line, which is dependent on GM-CSF (or interleukin 3) for continued growth. In vitro clonogenic assays indicated that scFv-GM-CSF could selectively induce growth inhibition of SKNBE cells by murine lymphoid cells.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals-
dc.subject.meshCytokines-
dc.subject.meshHumans-
dc.subject.meshImmunotherapy-
dc.subject.meshInterleukin-2-
dc.subject.meshMammals-
dc.subject.meshNeuroblastoma-
dc.subject.meshRecombinant Proteins-
dc.subject.meshTumor Cells, Cultured-
dc.titleTargeted cytokine delivery to neuroblastoma.en
dc.typeArticleen
dc.contributor.departmentMedical Oncology, Paterson Institute for Cancer Research, Wilmslow Road, Withington, Manchester M20 4BX, UK.en
dc.identifier.journalBiochemical Society Transactionsen
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