Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.

2.50
Hdl Handle:
http://hdl.handle.net/10541/83555
Title:
Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.
Authors:
Anderson, Mark; Ng, Szu Shien; Marchesi, Vanessa; MacIver, Fiona H; Stevens, Frances E; Riddell, Tracy; Glover, David M; Hagan, Iain M; McInerny, Christopher J
Abstract:
The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15(+) promoter that we call a PCB (pombe cell cycle box), which confers M-G(1)-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12(+), cdc19(+), fin1(+), sid2(+), ppb1(+), mid1(+)/dmf1(+) and plo1(+), which we find to be transcribed at M-G(1). A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
Affiliation:
Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, UK.
Citation:
Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. 2002, 21 (21):5745-55 EMBO J.
Journal:
The EMBO Journal
Issue Date:
1-Nov-2002
URI:
http://hdl.handle.net/10541/83555
DOI:
10.1093/emboj/cdf564
PubMed ID:
12411492
Type:
Article
Language:
en
ISSN:
0261-4189
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAnderson, Mark-
dc.contributor.authorNg, Szu Shien-
dc.contributor.authorMarchesi, Vanessa-
dc.contributor.authorMacIver, Fiona H-
dc.contributor.authorStevens, Frances E-
dc.contributor.authorRiddell, Tracy-
dc.contributor.authorGlover, David M-
dc.contributor.authorHagan, Iain M-
dc.contributor.authorMcInerny, Christopher J-
dc.date.accessioned2009-10-05T14:15:06Z-
dc.date.available2009-10-05T14:15:06Z-
dc.date.issued2002-11-01-
dc.identifier.citationPlo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. 2002, 21 (21):5745-55 EMBO J.en
dc.identifier.issn0261-4189-
dc.identifier.pmid12411492-
dc.identifier.doi10.1093/emboj/cdf564-
dc.identifier.urihttp://hdl.handle.net/10541/83555-
dc.description.abstractThe regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15(+) promoter that we call a PCB (pombe cell cycle box), which confers M-G(1)-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12(+), cdc19(+), fin1(+), sid2(+), ppb1(+), mid1(+)/dmf1(+) and plo1(+), which we find to be transcribed at M-G(1). A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.en
dc.language.isoenen
dc.subject.meshBase Sequence-
dc.subject.meshDNA Primers-
dc.subject.meshG1 Phase-
dc.subject.meshGenes, Fungal-
dc.subject.meshMitosis-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshTranscription, Genetic-
dc.titlePlo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.en
dc.typeArticleen
dc.contributor.departmentDivision of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, UK.en
dc.identifier.journalThe EMBO Journalen

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