Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.

2.50
Hdl Handle:
http://hdl.handle.net/10541/83550
Title:
Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.
Authors:
Grallert, Agnes; Hagan, Iain M
Abstract:
The Aspergillus nidulans protein kinase NIMA regulates mitotic commitment, while the human and Xenopus equivalents influence centrosome function. Two recessive, temperature-sensitive mutations in the Schizosaccharomyces pombe NIMA homologue, Fin1, blocked spindle formation at 37 degrees C. One of the two spindle pole bodies (SPBs) failed to nucleate microtubules. This phenotype was reduced by accelerating mitotic commitment through genetic inhibition of Wee1 or activation of either Cdc25 or Cdc2. Polo kinase (Plo1) normally associates with the SPB of mitotic, but not interphase cells. cut12.s11 is a dominant mutation in an SPB component that both suppresses cdc25 mutants and promotes Plo1 association with the interphase SPB. Both cut12.s11 phenotypes were abolished by removing Fin1 function. Elevating Fin1 levels promoted Plo1 recruitment to the interphase SPB of wild-type cells and reduced the severity of the cdc25.22 phenotype. These data are consistent with Fin1 regulating Plo1 function during mitotic commitment. The fin1 mitotic commitment and spindle phenotypes resemble distinct nimA phenotypes in different systems and suggest that the function of this family of kinases may be conserved across species.
Affiliation:
Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.
Citation:
Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB. 2002, 21 (12):3096-107 EMBO J.
Journal:
The EMBO Journal
Issue Date:
17-Jun-2002
URI:
http://hdl.handle.net/10541/83550
DOI:
10.1093/emboj/cdf294
PubMed ID:
12065422
Type:
Article
Language:
en
ISSN:
0261-4189
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGrallert, Agnes-
dc.contributor.authorHagan, Iain M-
dc.date.accessioned2009-10-05T15:51:44Z-
dc.date.available2009-10-05T15:51:44Z-
dc.date.issued2002-06-17-
dc.identifier.citationSchizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB. 2002, 21 (12):3096-107 EMBO J.en
dc.identifier.issn0261-4189-
dc.identifier.pmid12065422-
dc.identifier.doi10.1093/emboj/cdf294-
dc.identifier.urihttp://hdl.handle.net/10541/83550-
dc.description.abstractThe Aspergillus nidulans protein kinase NIMA regulates mitotic commitment, while the human and Xenopus equivalents influence centrosome function. Two recessive, temperature-sensitive mutations in the Schizosaccharomyces pombe NIMA homologue, Fin1, blocked spindle formation at 37 degrees C. One of the two spindle pole bodies (SPBs) failed to nucleate microtubules. This phenotype was reduced by accelerating mitotic commitment through genetic inhibition of Wee1 or activation of either Cdc25 or Cdc2. Polo kinase (Plo1) normally associates with the SPB of mitotic, but not interphase cells. cut12.s11 is a dominant mutation in an SPB component that both suppresses cdc25 mutants and promotes Plo1 association with the interphase SPB. Both cut12.s11 phenotypes were abolished by removing Fin1 function. Elevating Fin1 levels promoted Plo1 recruitment to the interphase SPB of wild-type cells and reduced the severity of the cdc25.22 phenotype. These data are consistent with Fin1 regulating Plo1 function during mitotic commitment. The fin1 mitotic commitment and spindle phenotypes resemble distinct nimA phenotypes in different systems and suggest that the function of this family of kinases may be conserved across species.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Cycle-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshDrosophila Proteins-
dc.subject.meshHumans-
dc.subject.meshMicrotubule-Associated Proteins-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshModels, Biological-
dc.subject.meshNuclear Proteins-
dc.subject.meshPhosphoproteins-
dc.subject.meshProtein-Serine-Threonine Kinases-
dc.subject.meshRecombinant Fusion Proteins-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.titleSchizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.en
dc.identifier.journalThe EMBO Journalen

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