The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82451
Title:
The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research.
Authors:
Eyden, Brian P
Abstract:
Some interpretational problems associated with the myofibroblast, which affect how this cell is identified, are discussed. Questions addressed include distinguishing between "external" lamina ("basement membrane") and the fibronectin fibril of the fibronexus; the nature of stress fibers (bundles of smooth-muscle myofilaments with focal densities); the utility of some of these features to distinguish between myofibroblastic and smooth-muscle cell surfaces; and cytoskeletal immunophenotype. The following points are emphasized. Myofibroblasts have a surface characterized by prominent fibronectin fibrils and fibronexus junctions, which are distinct from lamina ("basement membrane"). This can permit a distinction to be made between smooth-muscle and myofibroblastic lesions and tumors. Myofibroblasts are typically positive for vimentin and alpha-smooth-muscle actin, but desmin is not a useful discriminant between smooth-muscle and myofibroblastic lesions. The main features for defining the myofibroblast are abundant rough endoplasmic reticulum; modestly developed peripheral myofilaments with focal densities (stress fibers); fibronexus junctions; vimentin and smooth-muscle actin immunostaining. Other features include a Golgi apparatus and collagen secretion granules, gap junctions, and actin-associated nondesmosomal junctions. Illustrations of the usefulness of these criteria in the diagnosis of soft-tissue lesions (myofibrosarcoma, so-called myofibroblastoma) are given.
Affiliation:
Department of Histopathology, Christie Hospital NHS Trust, Manchester, United Kingdom. Brian.Eyden@christie-tr.nwest.nhs.uk
Citation:
The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research., 25 (1):39-50 Ultrastruct Pathol
Journal:
Ultrastructural Pathology
Issue Date:
2001
URI:
http://hdl.handle.net/10541/82451
PubMed ID:
11297318
Type:
Article
Language:
en
ISSN:
0191-3123
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorEyden, Brian P-
dc.date.accessioned2009-09-24T11:15:08Z-
dc.date.available2009-09-24T11:15:08Z-
dc.date.issued2001-
dc.identifier.citationThe myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research., 25 (1):39-50 Ultrastruct Patholen
dc.identifier.issn0191-3123-
dc.identifier.pmid11297318-
dc.identifier.urihttp://hdl.handle.net/10541/82451-
dc.description.abstractSome interpretational problems associated with the myofibroblast, which affect how this cell is identified, are discussed. Questions addressed include distinguishing between "external" lamina ("basement membrane") and the fibronectin fibril of the fibronexus; the nature of stress fibers (bundles of smooth-muscle myofilaments with focal densities); the utility of some of these features to distinguish between myofibroblastic and smooth-muscle cell surfaces; and cytoskeletal immunophenotype. The following points are emphasized. Myofibroblasts have a surface characterized by prominent fibronectin fibrils and fibronexus junctions, which are distinct from lamina ("basement membrane"). This can permit a distinction to be made between smooth-muscle and myofibroblastic lesions and tumors. Myofibroblasts are typically positive for vimentin and alpha-smooth-muscle actin, but desmin is not a useful discriminant between smooth-muscle and myofibroblastic lesions. The main features for defining the myofibroblast are abundant rough endoplasmic reticulum; modestly developed peripheral myofilaments with focal densities (stress fibers); fibronexus junctions; vimentin and smooth-muscle actin immunostaining. Other features include a Golgi apparatus and collagen secretion granules, gap junctions, and actin-associated nondesmosomal junctions. Illustrations of the usefulness of these criteria in the diagnosis of soft-tissue lesions (myofibrosarcoma, so-called myofibroblastoma) are given.en
dc.language.isoenen
dc.subject.meshActins-
dc.subject.meshBasement Membrane-
dc.subject.meshCell Surface Extensions-
dc.subject.meshCytoskeleton-
dc.subject.meshEndoplasmic Reticulum, Rough-
dc.subject.meshFibroblasts-
dc.subject.meshFibronectins-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIntercellular Junctions-
dc.subject.meshMicrofilaments-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMuscle, Smooth-
dc.subject.meshVimentin-
dc.titleThe myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research.en
dc.typeArticleen
dc.contributor.departmentDepartment of Histopathology, Christie Hospital NHS Trust, Manchester, United Kingdom. Brian.Eyden@christie-tr.nwest.nhs.uken
dc.identifier.journalUltrastructural Pathologyen
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