2.50
Hdl Handle:
http://hdl.handle.net/10541/82444
Title:
A role for cell polarity proteins in mitotic exit.
Authors:
Höfken, Thomas; Schiebel, Elmar
Abstract:
The budding yeast mitotic exit network (MEN) is a signal transduction cascade that controls exit from mitosis by facilitating the release of the cell cycle phosphatase Cdc14 from the nucleolus. The G protein Tem1 regulates MEN activity. The Tem1 guanine nucleotide exchange factor (GEF) Lte1 associates with the cortex of the bud and activates the MEN upon the formation of an anaphase spindle. Thus, the cell cortex has an important but ill-defined role in MEN regulation. Here, we describe a network of conserved cortical cell polarity proteins that have key roles in mitotic exit. The Rho-like GTPase Cdc42, its GEF Cdc24 and its effector Cla4 [a member of the p21-activated kinases (PAKs)] control the initial binding and activation of Lte1 to the bud cortex. Moreover, Cdc24, Cdc42 and Ste20, another PAK, probably function parallel to Lte1 in facilitating mitotic exit. Finally, the cell polarity proteins Kel1 and Kel2 are present in complexes with both Lte1 and Tem1, and negatively regulate mitotic exit.
Affiliation:
The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
Citation:
A role for cell polarity proteins in mitotic exit. 2002, 21 (18):4851-62 EMBO J.
Journal:
The EMBO Journal
Issue Date:
16-Sep-2002
URI:
http://hdl.handle.net/10541/82444
DOI:
10.1093/emboj/cdf481
PubMed ID:
12234925
Type:
Article
Language:
en
ISSN:
0261-4189
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHöfken, Thomas-
dc.contributor.authorSchiebel, Elmar-
dc.date.accessioned2009-09-24T10:06:17Z-
dc.date.available2009-09-24T10:06:17Z-
dc.date.issued2002-09-16-
dc.identifier.citationA role for cell polarity proteins in mitotic exit. 2002, 21 (18):4851-62 EMBO J.en
dc.identifier.issn0261-4189-
dc.identifier.pmid12234925-
dc.identifier.doi10.1093/emboj/cdf481-
dc.identifier.urihttp://hdl.handle.net/10541/82444-
dc.description.abstractThe budding yeast mitotic exit network (MEN) is a signal transduction cascade that controls exit from mitosis by facilitating the release of the cell cycle phosphatase Cdc14 from the nucleolus. The G protein Tem1 regulates MEN activity. The Tem1 guanine nucleotide exchange factor (GEF) Lte1 associates with the cortex of the bud and activates the MEN upon the formation of an anaphase spindle. Thus, the cell cortex has an important but ill-defined role in MEN regulation. Here, we describe a network of conserved cortical cell polarity proteins that have key roles in mitotic exit. The Rho-like GTPase Cdc42, its GEF Cdc24 and its effector Cla4 [a member of the p21-activated kinases (PAKs)] control the initial binding and activation of Lte1 to the bud cortex. Moreover, Cdc24, Cdc42 and Ste20, another PAK, probably function parallel to Lte1 in facilitating mitotic exit. Finally, the cell polarity proteins Kel1 and Kel2 are present in complexes with both Lte1 and Tem1, and negatively regulate mitotic exit.en
dc.language.isoenen
dc.subject.meshCaenorhabditis elegans Proteins-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCell Polarity-
dc.subject.meshFungal Proteins-
dc.subject.meshGuanine Nucleotide Exchange Factors-
dc.subject.meshMacromolecular Substances-
dc.subject.meshMitosis-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein-Serine-Threonine Kinases-
dc.subject.meshProteins-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshSignal Transduction-
dc.subject.meshTemperature-
dc.subject.meshcdc42 GTP-Binding Protein, Saccharomyces cerevisiae-
dc.titleA role for cell polarity proteins in mitotic exit.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.en
dc.identifier.journalThe EMBO Journalen

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