Collagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82437
Title:
Collagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study.
Authors:
Haylett, Ann K; Higley, Katherine; Chiu, Maybo; Shackley, David C; Moore, James V
Abstract:
Photodynamic therapy (PDT) has been associated anecdotally with good quality healing and an absence of scar formation. Our previous studies, examining the levels of the collagen specific molecular chaperone Hsp47, have noted differences in the response after photodynamic therapy and hyperthermia at both the transcriptional and translational levels. In the present study the levels of Hsp47 after exposure to two chemotherapeutic agents (bleomycin and mitomycin). ionising radiation, hyperthermia and haematoporphyrin ester (HpE) mediated PDT were compared in both mouse and human fibroblast cell lines. A rapid assay for soluble collagen has also been used to quantify soluble collagen levels at early time points after treatment. Peak Hsp47 levels were found to correlate well with peak collagen levels. The results show that the levels of collagen measured in vitro are elevated in modalities associated with scarring in vivo but not after HpE-PDT.
Affiliation:
Cancer Research-UK Laser Oncology Group, Paterson Institute for Cancer Research, Wilmslow Rd, Manchester, UK M20 4BX. ahaylett@picr.man.ac.uk
Citation:
Collagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study. 2002, 1 (9):673-7 Photochem. Photobiol. Sci.
Journal:
Photochemical & Photobiological Sciences
Issue Date:
Sep-2002
URI:
http://hdl.handle.net/10541/82437
DOI:
10.1039/b203344e
PubMed ID:
12665304
Type:
Article
Language:
en
ISSN:
1474-905X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHaylett, Ann K-
dc.contributor.authorHigley, Katherine-
dc.contributor.authorChiu, Maybo-
dc.contributor.authorShackley, David C-
dc.contributor.authorMoore, James V-
dc.date.accessioned2009-09-24T08:39:58Z-
dc.date.available2009-09-24T08:39:58Z-
dc.date.issued2002-09-
dc.identifier.citationCollagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study. 2002, 1 (9):673-7 Photochem. Photobiol. Sci.en
dc.identifier.issn1474-905X-
dc.identifier.pmid12665304-
dc.identifier.doi10.1039/b203344e-
dc.identifier.urihttp://hdl.handle.net/10541/82437-
dc.description.abstractPhotodynamic therapy (PDT) has been associated anecdotally with good quality healing and an absence of scar formation. Our previous studies, examining the levels of the collagen specific molecular chaperone Hsp47, have noted differences in the response after photodynamic therapy and hyperthermia at both the transcriptional and translational levels. In the present study the levels of Hsp47 after exposure to two chemotherapeutic agents (bleomycin and mitomycin). ionising radiation, hyperthermia and haematoporphyrin ester (HpE) mediated PDT were compared in both mouse and human fibroblast cell lines. A rapid assay for soluble collagen has also been used to quantify soluble collagen levels at early time points after treatment. Peak Hsp47 levels were found to correlate well with peak collagen levels. The results show that the levels of collagen measured in vitro are elevated in modalities associated with scarring in vivo but not after HpE-PDT.en
dc.language.isoenen
dc.subjectHaematoporphyrinsen
dc.subject.meshAnimals-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshBleomycin-
dc.subject.meshCell Line-
dc.subject.meshCicatrix-
dc.subject.meshCollagen-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshFibroblasts-
dc.subject.meshGamma Rays-
dc.subject.meshHSP47 Heat-Shock Proteins-
dc.subject.meshHeat-Shock Proteins-
dc.subject.meshHematoporphyrins-
dc.subject.meshHot Temperature-
dc.subject.meshKinetics-
dc.subject.meshMice-
dc.subject.meshMitomycin-
dc.subject.meshPhotochemotherapy-
dc.titleCollagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study.en
dc.typeArticleen
dc.contributor.departmentCancer Research-UK Laser Oncology Group, Paterson Institute for Cancer Research, Wilmslow Rd, Manchester, UK M20 4BX. ahaylett@picr.man.ac.uken
dc.identifier.journalPhotochemical & Photobiological Sciencesen
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