DNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82345
Title:
DNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study.
Authors:
Haylett, Ann K; Ward, Timothy H; Moore, James V
Abstract:
Using normal, untransformed, human fibroblasts, the effectiveness of aminolevulinic (ALA)-mediated photodynamic therapy (PDT) was investigated in terms of both clonogenic survival and DNA damage. The response of normal fibroblasts was then compared with Gorlin syndrome-derived fibroblasts (basal cell nevus syndrome [BCNS]). In terms of clonogenic survival, no significant differences were observed between the two groups of cells. Using the alkaline comet assay, initial DNA damage after PDT was measured. Some DNA damage was detected at higher doses, but this was fully repaired within 24 h of treatment. The BCNS-derived cells showed levels of initial damage that did not differ significantly from normal lines.
Affiliation:
Cancer Research-UK Laser Oncology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. ahaylett@picr.man.ac.uk
Citation:
DNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study. 2003, 78 (4):337-41 Photochem. Photobiol.
Journal:
Photochemistry and Photobiology
Issue Date:
Oct-2003
URI:
http://hdl.handle.net/10541/82345
PubMed ID:
14626660
Type:
Article
Language:
en
ISSN:
0031-8655
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHaylett, Ann K-
dc.contributor.authorWard, Timothy H-
dc.contributor.authorMoore, James V-
dc.date.accessioned2009-09-23T14:00:53Z-
dc.date.available2009-09-23T14:00:53Z-
dc.date.issued2003-10-
dc.identifier.citationDNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study. 2003, 78 (4):337-41 Photochem. Photobiol.en
dc.identifier.issn0031-8655-
dc.identifier.pmid14626660-
dc.identifier.urihttp://hdl.handle.net/10541/82345-
dc.description.abstractUsing normal, untransformed, human fibroblasts, the effectiveness of aminolevulinic (ALA)-mediated photodynamic therapy (PDT) was investigated in terms of both clonogenic survival and DNA damage. The response of normal fibroblasts was then compared with Gorlin syndrome-derived fibroblasts (basal cell nevus syndrome [BCNS]). In terms of clonogenic survival, no significant differences were observed between the two groups of cells. Using the alkaline comet assay, initial DNA damage after PDT was measured. Some DNA damage was detected at higher doses, but this was fully repaired within 24 h of treatment. The BCNS-derived cells showed levels of initial damage that did not differ significantly from normal lines.en
dc.language.isoenen
dc.subject.meshAminolevulinic Acid-
dc.subject.meshBasal Cell Nevus Syndrome-
dc.subject.meshCell Survival-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshFibroblasts-
dc.subject.meshHumans-
dc.subject.meshPhotochemotherapy-
dc.titleDNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study.en
dc.typeArticleen
dc.contributor.departmentCancer Research-UK Laser Oncology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. ahaylett@picr.man.ac.uken
dc.identifier.journalPhotochemistry and Photobiologyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.