Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82299
Title:
Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.
Authors:
Maekawa, Hiromi; Usui, Takeo; Knop, Michael; Schiebel, Elmar
Abstract:
The budding yeast spindle aligns along the mother- bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1, mediates contacts of CMs with the cortex of the daughter cell, the bud. Here we established a novel series of events that target Kar9 to the bud cortex. First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9. Thirdly, Kar9 and Cdc28 then move from the SPB to the plus end of CMs directed towards the bud. This movement is dependent upon the microtubule motor protein Kip2. Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles.
Affiliation:
The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
Citation:
Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. 2003, 22 (3):438-49 EMBO J.
Journal:
The EMBO Journal
Issue Date:
3-Feb-2003
URI:
http://hdl.handle.net/10541/82299
DOI:
10.1093/emboj/cdg063
PubMed ID:
12554645
Type:
Article
Language:
en
ISSN:
0261-4189
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMaekawa, Hiromi-
dc.contributor.authorUsui, Takeo-
dc.contributor.authorKnop, Michael-
dc.contributor.authorSchiebel, Elmar-
dc.date.accessioned2009-09-23T11:57:59Z-
dc.date.available2009-09-23T11:57:59Z-
dc.date.issued2003-02-03-
dc.identifier.citationYeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. 2003, 22 (3):438-49 EMBO J.en
dc.identifier.issn0261-4189-
dc.identifier.pmid12554645-
dc.identifier.doi10.1093/emboj/cdg063-
dc.identifier.urihttp://hdl.handle.net/10541/82299-
dc.description.abstractThe budding yeast spindle aligns along the mother- bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1, mediates contacts of CMs with the cortex of the daughter cell, the bud. Here we established a novel series of events that target Kar9 to the bud cortex. First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9. Thirdly, Kar9 and Cdc28 then move from the SPB to the plus end of CMs directed towards the bud. This movement is dependent upon the microtubule motor protein Kip2. Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles.en
dc.language.isoenen
dc.subject.meshCDC2 Protein Kinase-
dc.subject.meshCDC28 Protein Kinase, S cerevisiae-
dc.subject.meshCell Cycle-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshMicrotubule Proteins-
dc.subject.meshMicrotubules-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshNuclear Proteins-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Binding-
dc.subject.meshProtein Transport-
dc.subject.meshRecombinant Fusion Proteins-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshTwo-Hybrid System Techniques-
dc.titleYeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.en
dc.identifier.journalThe EMBO Journalen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.