The conserved Nup107-160 complex is critical for nuclear pore complex assembly.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82256
Title:
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
Authors:
Walther, Tobias C; Alves, Annabelle; Pickersgill, Helen; Loïodice, Isabelle; Hetzer, Martin; Galy, Vincent; Hülsmann, Bastian B; Köcher, Thomas; Wilm, Matthias; Allen, Terence D; Mattaj, Iain W; Doye, Valérie
Abstract:
Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.
Affiliation:
EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Citation:
The conserved Nup107-160 complex is critical for nuclear pore complex assembly. 2003, 113 (2):195-206 Cell
Journal:
Cell
Issue Date:
18-Apr-2003
URI:
http://hdl.handle.net/10541/82256
PubMed ID:
12705868
Type:
Article
Language:
en
ISSN:
0092-8674
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWalther, Tobias C-
dc.contributor.authorAlves, Annabelle-
dc.contributor.authorPickersgill, Helen-
dc.contributor.authorLoïodice, Isabelle-
dc.contributor.authorHetzer, Martin-
dc.contributor.authorGaly, Vincent-
dc.contributor.authorHülsmann, Bastian B-
dc.contributor.authorKöcher, Thomas-
dc.contributor.authorWilm, Matthias-
dc.contributor.authorAllen, Terence D-
dc.contributor.authorMattaj, Iain W-
dc.contributor.authorDoye, Valérie-
dc.date.accessioned2009-09-23T10:24:23Z-
dc.date.available2009-09-23T10:24:23Z-
dc.date.issued2003-04-18-
dc.identifier.citationThe conserved Nup107-160 complex is critical for nuclear pore complex assembly. 2003, 113 (2):195-206 Cellen
dc.identifier.issn0092-8674-
dc.identifier.pmid12705868-
dc.identifier.urihttp://hdl.handle.net/10541/82256-
dc.description.abstractNuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Extracts-
dc.subject.meshChelating Agents-
dc.subject.meshChromatin-
dc.subject.meshEukaryotic Cells-
dc.subject.meshFemale-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshHela Cells-
dc.subject.meshHumans-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshNuclear Pore-
dc.subject.meshNuclear Pore Complex Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshOocytes-
dc.subject.meshPhenotype-
dc.subject.meshXenopus Proteins-
dc.subject.meshXenopus laevis-
dc.titleThe conserved Nup107-160 complex is critical for nuclear pore complex assembly.en
dc.typeArticleen
dc.contributor.departmentEMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.en
dc.identifier.journalCellen

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