Identification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82255
Title:
Identification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs.
Authors:
Stringer, Sally E; Nelson, Matthew S; Gupta, Pankaj
Abstract:
We previously showed that heparan sulfate (HS) is required for in vitro cytokine + chemokine-mediated maintenance of primitive human hematopoietic progenitors. However, HS preparations are mixtures of polysaccharide chains of varying size, structure, and protein-binding abilities. Therefore, we examined whether the long-term culture-initiating cells (LTC-IC) supportive capability of HS is attributable to an oligosaccharide of defined length and protein-binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-IC maintenance in the presence of low-dose cytokines and a single chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), was examined. LTC-IC supportive capability of HS oligosaccharides correlated directly with size and MIP-1alpha binding ability. A specific MIP-1alpha-binding HS oligosaccharide preparation of M(r) 10 kDa that optimally supported LTC-IC maintenance was identified. This oligosaccharide had the structure required for MIP-1alpha binding, which we have recently described. The present study defines the minimum size and structural features of LTC-IC supportive HS.
Affiliation:
Paterson Institute for Cancer Research, Manchester, United Kingdom.
Citation:
Identification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs. 2003, 101 (6):2243-5 Blood
Journal:
Blood
Issue Date:
15-Mar-2003
URI:
http://hdl.handle.net/10541/82255
DOI:
10.1182/blood-2002-08-2588
PubMed ID:
12406885
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorStringer, Sally E-
dc.contributor.authorNelson, Matthew S-
dc.contributor.authorGupta, Pankaj-
dc.date.accessioned2009-09-23T10:22:54Z-
dc.date.available2009-09-23T10:22:54Z-
dc.date.issued2003-03-15-
dc.identifier.citationIdentification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs. 2003, 101 (6):2243-5 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid12406885-
dc.identifier.doi10.1182/blood-2002-08-2588-
dc.identifier.urihttp://hdl.handle.net/10541/82255-
dc.description.abstractWe previously showed that heparan sulfate (HS) is required for in vitro cytokine + chemokine-mediated maintenance of primitive human hematopoietic progenitors. However, HS preparations are mixtures of polysaccharide chains of varying size, structure, and protein-binding abilities. Therefore, we examined whether the long-term culture-initiating cells (LTC-IC) supportive capability of HS is attributable to an oligosaccharide of defined length and protein-binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-IC maintenance in the presence of low-dose cytokines and a single chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), was examined. LTC-IC supportive capability of HS oligosaccharides correlated directly with size and MIP-1alpha binding ability. A specific MIP-1alpha-binding HS oligosaccharide preparation of M(r) 10 kDa that optimally supported LTC-IC maintenance was identified. This oligosaccharide had the structure required for MIP-1alpha binding, which we have recently described. The present study defines the minimum size and structural features of LTC-IC supportive HS.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCells, Cultured-
dc.subject.meshChemokine CCL3-
dc.subject.meshChemokine CCL4-
dc.subject.meshHLA-DR Antigens-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshHumans-
dc.subject.meshMacrophage Inflammatory Proteins-
dc.subject.meshOligosaccharides-
dc.subject.meshProtein Binding-
dc.titleIdentification of an MIP-1alpha -binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-ICs.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalBlooden
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