No alteration in NK function or zeta chain expression in NK and T cells of cervical cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82153
Title:
No alteration in NK function or zeta chain expression in NK and T cells of cervical cancer patients.
Authors:
Onon, Toli S; Kitchener, Henry C; Duggan-Keen, Margaret F; Stern, Peter L
Abstract:
OBJECTIVE: To investigate in vitro natural killer (NK) cell activity and expression of signal-transducing zeta chains in patients with cervical cancer. PATIENTS AND METHODS: Experiments were performed with frozen lymphocytes from patients at all disease stages and from healthy controls. Thawed NK were activated by overnight incubation in interferon-gamma (IFN-gamma); activity against two target cell lines was assessed by 4-h (51)Cr release assay. Targets chosen were K562, an erythroleukemic cell line, and a cervical carcinoma cell line designated 808. T and NK cell zeta chain expression was measured by flow cytometry. RESULTS: Patients' NK were found to be as cytotoxic as those of normal controls against cell lines K562 and 808. Patient T and NK cells did not show significant down-regulation of the zeta chain. CONCLUSIONS: We have found no evidence to suggest that loss of zeta chains is a mechanism for immunocompromise in patients with cervical carcinoma. IFN-recoverable patient NK activity is not reduced compared to matched controls. This may be clinically relevant since NK are active against cells exhibiting class I human leukocyte antigen (HLA) down-regulation and many cervical cancers show loss of HLA.
Affiliation:
Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom. toli.onon@srht.nhs.uk
Citation:
No alteration in NK function or zeta chain expression in NK and T cells of cervical cancer patients. 2003, 89 (1):120-8 Gynecol. Oncol.
Journal:
Gynecologic Oncology
Issue Date:
Apr-2003
URI:
http://hdl.handle.net/10541/82153
DOI:
10.1016/S0090-8258(03)00009-X
PubMed ID:
12694665
Type:
Article
Language:
en
ISSN:
0090-8258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorOnon, Toli S-
dc.contributor.authorKitchener, Henry C-
dc.contributor.authorDuggan-Keen, Margaret F-
dc.contributor.authorStern, Peter L-
dc.date.accessioned2009-09-22T16:18:17Z-
dc.date.available2009-09-22T16:18:17Z-
dc.date.issued2003-04-
dc.identifier.citationNo alteration in NK function or zeta chain expression in NK and T cells of cervical cancer patients. 2003, 89 (1):120-8 Gynecol. Oncol.en
dc.identifier.issn0090-8258-
dc.identifier.pmid12694665-
dc.identifier.doi10.1016/S0090-8258(03)00009-X-
dc.identifier.urihttp://hdl.handle.net/10541/82153-
dc.description.abstractOBJECTIVE: To investigate in vitro natural killer (NK) cell activity and expression of signal-transducing zeta chains in patients with cervical cancer. PATIENTS AND METHODS: Experiments were performed with frozen lymphocytes from patients at all disease stages and from healthy controls. Thawed NK were activated by overnight incubation in interferon-gamma (IFN-gamma); activity against two target cell lines was assessed by 4-h (51)Cr release assay. Targets chosen were K562, an erythroleukemic cell line, and a cervical carcinoma cell line designated 808. T and NK cell zeta chain expression was measured by flow cytometry. RESULTS: Patients' NK were found to be as cytotoxic as those of normal controls against cell lines K562 and 808. Patient T and NK cells did not show significant down-regulation of the zeta chain. CONCLUSIONS: We have found no evidence to suggest that loss of zeta chains is a mechanism for immunocompromise in patients with cervical carcinoma. IFN-recoverable patient NK activity is not reduced compared to matched controls. This may be clinically relevant since NK are active against cells exhibiting class I human leukocyte antigen (HLA) down-regulation and many cervical cancers show loss of HLA.en
dc.language.isoenen
dc.subjectCancer Stagingen
dc.subjectUterine Cervical Canceren
dc.subject.meshAge Factors-
dc.subject.meshAntigens, CD3-
dc.subject.meshChromium Radioisotopes-
dc.subject.meshCytotoxicity, Immunologic-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshInterferon-gamma-
dc.subject.meshK562 Cells-
dc.subject.meshKiller Cells, Natural-
dc.subject.meshMiddle Aged-
dc.subject.meshNeoplasm Staging-
dc.subject.meshReceptors, IgG-
dc.subject.meshT-Lymphocytes-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleNo alteration in NK function or zeta chain expression in NK and T cells of cervical cancer patients.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom. toli.onon@srht.nhs.uken
dc.identifier.journalGynecologic Oncologyen

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