Identification of a putative intestinal stem cell and early lineage marker; musashi-1.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82128
Title:
Identification of a putative intestinal stem cell and early lineage marker; musashi-1.
Authors:
Potten, Christopher S; Booth, Catherine; Tudor, Gregory L; Booth, Dawn; Brady, Ged; Hurley, Patricia; Ashton, Garry; Clarke, Robert B; Sakakibara, Shin-ichi; Okano, Hideyuki
Abstract:
There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / - ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive. In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.
Affiliation:
Epithelial Biology Department, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. c.potten@epistem.co.uk
Citation:
Identification of a putative intestinal stem cell and early lineage marker; musashi-1. 2003, 71 (1):28-41 Differentiation
Journal:
Differentiation
Issue Date:
Jan-2003
URI:
http://hdl.handle.net/10541/82128
PubMed ID:
12558601
Type:
Article
Language:
en
ISSN:
0301-4681
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPotten, Christopher S-
dc.contributor.authorBooth, Catherine-
dc.contributor.authorTudor, Gregory L-
dc.contributor.authorBooth, Dawn-
dc.contributor.authorBrady, Ged-
dc.contributor.authorHurley, Patricia-
dc.contributor.authorAshton, Garry-
dc.contributor.authorClarke, Robert B-
dc.contributor.authorSakakibara, Shin-ichi-
dc.contributor.authorOkano, Hideyuki-
dc.date.accessioned2009-09-22T15:45:58Z-
dc.date.available2009-09-22T15:45:58Z-
dc.date.issued2003-01-
dc.identifier.citationIdentification of a putative intestinal stem cell and early lineage marker; musashi-1. 2003, 71 (1):28-41 Differentiationen
dc.identifier.issn0301-4681-
dc.identifier.pmid12558601-
dc.identifier.urihttp://hdl.handle.net/10541/82128-
dc.description.abstractThere are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / - ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive. In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.en
dc.language.isoenen
dc.subjectIntestinal Canceren
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAnimals-
dc.subject.meshBiological Markers-
dc.subject.meshCell Lineage-
dc.subject.meshChild-
dc.subject.meshGamma Rays-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIntestinal Neoplasms-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshNerve Tissue Proteins-
dc.subject.meshRNA-Binding Proteins-
dc.subject.meshStem Cells-
dc.titleIdentification of a putative intestinal stem cell and early lineage marker; musashi-1.en
dc.typeArticleen
dc.contributor.departmentEpithelial Biology Department, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. c.potten@epistem.co.uken
dc.identifier.journalDifferentiationen

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