The human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82125
Title:
The human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence.
Authors:
Slater, Nicholas J; Yamaguchi, Masafumi; Rothwell, Dominic G; Baker, Patrick; Heyworth, Clare M; Chopra, Rajesh
Abstract:
A number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the alpha1 and alpha2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of alpha1 or alpha2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-alpha2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-alpha1-expressing cells.
Affiliation:
Stem Cell and Leukaemia Biology Group, Christie Hospital, Manchester, UK.
Citation:
The human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence. 2003, 122 (1):150-8 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Jul-2003
URI:
http://hdl.handle.net/10541/82125
PubMed ID:
12823357
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSlater, Nicholas J-
dc.contributor.authorYamaguchi, Masafumi-
dc.contributor.authorRothwell, Dominic G-
dc.contributor.authorBaker, Patrick-
dc.contributor.authorHeyworth, Clare M-
dc.contributor.authorChopra, Rajesh-
dc.date.accessioned2009-09-22T15:11:14Z-
dc.date.available2009-09-22T15:11:14Z-
dc.date.issued2003-07-
dc.identifier.citationThe human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence. 2003, 122 (1):150-8 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid12823357-
dc.identifier.urihttp://hdl.handle.net/10541/82125-
dc.description.abstractA number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the alpha1 and alpha2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of alpha1 or alpha2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-alpha2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-alpha1-expressing cells.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAlternative Splicing-
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD34-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshErythroid Precursor Cells-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshProtein Isoforms-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, Granulocyte-Macrophage Colony-Stimulating Factor-
dc.titleThe human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence.en
dc.typeArticleen
dc.contributor.departmentStem Cell and Leukaemia Biology Group, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Haematologyen

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