Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14.

2.50
Hdl Handle:
http://hdl.handle.net/10541/82119
Title:
Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14.
Authors:
Pereira, Gislene; Schiebel, Elmar
Abstract:
The inner centromere-like protein (INCENP) forms a complex with the evolutionarily conserved family of Aurora Bkinases. The INCENP-Aurora complex helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. INCENP-Aurora associates with kinetochores in metaphase and with spindle microtubules in anaphase, yet the trigger for this abrupt transfer is unknown. Here we show that the conserved phosphatase Cdc14 regulated the yeast INCENP-Aurora complex, Sli15-Ipl1. Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles. Activation of Cdc14 by separase was sufficient for Sli15 dephosphorylation and relocalization. Cdc14 not only regulates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.
Affiliation:
The Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.
Citation:
Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. 2003, 302 (5653):2120-4 Science
Journal:
Science
Issue Date:
19-Dec-2003
URI:
http://hdl.handle.net/10541/82119
DOI:
10.1126/science.1091936
PubMed ID:
14605209
Type:
Article
Language:
en
ISSN:
1095-9203
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPereira, Gislene-
dc.contributor.authorSchiebel, Elmar-
dc.date.accessioned2009-09-22T14:45:21Z-
dc.date.available2009-09-22T14:45:21Z-
dc.date.issued2003-12-19-
dc.identifier.citationSeparase regulates INCENP-Aurora B anaphase spindle function through Cdc14. 2003, 302 (5653):2120-4 Scienceen
dc.identifier.issn1095-9203-
dc.identifier.pmid14605209-
dc.identifier.doi10.1126/science.1091936-
dc.identifier.urihttp://hdl.handle.net/10541/82119-
dc.description.abstractThe inner centromere-like protein (INCENP) forms a complex with the evolutionarily conserved family of Aurora Bkinases. The INCENP-Aurora complex helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. INCENP-Aurora associates with kinetochores in metaphase and with spindle microtubules in anaphase, yet the trigger for this abrupt transfer is unknown. Here we show that the conserved phosphatase Cdc14 regulated the yeast INCENP-Aurora complex, Sli15-Ipl1. Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles. Activation of Cdc14 by separase was sufficient for Sli15 dephosphorylation and relocalization. Cdc14 not only regulates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.en
dc.language.isoenen
dc.subject.meshAnaphase-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCell Nucleolus-
dc.subject.meshChromosomal Proteins, Non-Histone-
dc.subject.meshEndopeptidases-
dc.subject.meshKinetochores-
dc.subject.meshMetaphase-
dc.subject.meshMicrotubule-Associated Proteins-
dc.subject.meshMicrotubules-
dc.subject.meshMitosis-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Binding-
dc.subject.meshProtein Kinases-
dc.subject.meshProtein Tyrosine Phosphatases-
dc.subject.meshProtein-Serine-Threonine Kinases-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.titleSeparase regulates INCENP-Aurora B anaphase spindle function through Cdc14.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.en
dc.identifier.journalScienceen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.