Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/81268
Title:
Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma.
Authors:
Lang, Shona H; Hyde, Catherine; Reid, Ian N; Hitchcock, Ian S; Hart, Claire A; Bryden, A A G; Villette, Jean-Marie; Stower, Michael J; Maitland, Norman J
Abstract:
BACKGROUND: The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers. METHODS: Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines and tissues was used to identify expression of cytokeratins 8 and 1, 5, 10, 14, vimentin, prostate specific antigen, prostate specific membrane antigen, androgen receptor, desmoglein, E-cadherin, beta1 integrin, CD44, hmet, vinculin and actin. RESULTS: Expression of vimentin was the only marker to correlate with motility, no markers correlated to invasion. Lower vimentin expression was observed in cells with low motility (PNT2-C2) and high expression in cells with high motility (P4E6, PNT1a, PC-3). Vimentin expression was not detected in well differentiated tumours, moderately differentiated tumours contained vimentin positive cells (1/9 bone scan negative, 2/5 bone scan positive), but the majority of poorly differentiated cancers (4/11 bone scan negative, 9/14 bone scan positive) and bone metastases (7/8) had high vimentin expression in tumour cells. CONCLUSIONS: Motile prostate cancer cell lines express vimentin. In tissue sections, the presence of vimentin positive tumour cells correlated positively to poorly differentiated cancers and the presence of bone metastases.
Affiliation:
YCR Cancer Research Unit, University of York, Heslington, York, United Kingdom. SHL@york.ac.uk
Citation:
Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma. 2002, 52 (4):253-63 Prostate
Journal:
The Prostate
Issue Date:
1-Sep-2002
URI:
http://hdl.handle.net/10541/81268
DOI:
10.1002/pros.10088
PubMed ID:
12210485
Type:
Article
Language:
en
ISSN:
0270-4137
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLang, Shona H-
dc.contributor.authorHyde, Catherine-
dc.contributor.authorReid, Ian N-
dc.contributor.authorHitchcock, Ian S-
dc.contributor.authorHart, Claire A-
dc.contributor.authorBryden, A A G-
dc.contributor.authorVillette, Jean-Marie-
dc.contributor.authorStower, Michael J-
dc.contributor.authorMaitland, Norman J-
dc.date.accessioned2009-09-16T11:44:39Z-
dc.date.available2009-09-16T11:44:39Z-
dc.date.issued2002-09-01-
dc.identifier.citationEnhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma. 2002, 52 (4):253-63 Prostateen
dc.identifier.issn0270-4137-
dc.identifier.pmid12210485-
dc.identifier.doi10.1002/pros.10088-
dc.identifier.urihttp://hdl.handle.net/10541/81268-
dc.description.abstractBACKGROUND: The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers. METHODS: Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines and tissues was used to identify expression of cytokeratins 8 and 1, 5, 10, 14, vimentin, prostate specific antigen, prostate specific membrane antigen, androgen receptor, desmoglein, E-cadherin, beta1 integrin, CD44, hmet, vinculin and actin. RESULTS: Expression of vimentin was the only marker to correlate with motility, no markers correlated to invasion. Lower vimentin expression was observed in cells with low motility (PNT2-C2) and high expression in cells with high motility (P4E6, PNT1a, PC-3). Vimentin expression was not detected in well differentiated tumours, moderately differentiated tumours contained vimentin positive cells (1/9 bone scan negative, 2/5 bone scan positive), but the majority of poorly differentiated cancers (4/11 bone scan negative, 9/14 bone scan positive) and bone metastases (7/8) had high vimentin expression in tumour cells. CONCLUSIONS: Motile prostate cancer cell lines express vimentin. In tissue sections, the presence of vimentin positive tumour cells correlated positively to poorly differentiated cancers and the presence of bone metastases.en
dc.language.isoenen
dc.subjectBone Canceren
dc.subjectProstatic Canceren
dc.subjectCultured Tumour Cellsen
dc.subjectBiological Tumour Markersen
dc.subject.meshBone Neoplasms-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Movement-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMale-
dc.subject.meshNeoplasm Invasiveness-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Markers, Biological-
dc.subject.meshVimentin-
dc.titleEnhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma.en
dc.typeArticleen
dc.contributor.departmentYCR Cancer Research Unit, University of York, Heslington, York, United Kingdom. SHL@york.ac.uken
dc.identifier.journalThe Prostateen
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