Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow.

2.50
Hdl Handle:
http://hdl.handle.net/10541/80228
Title:
Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow.
Authors:
Siebler, T; Robson, Helen; Bromley, Michael; Stevens, D A; Shalet, Stephen M; Williams, Graham R
Abstract:
Thyroid hormone (T(3)) plays a key role in endochondral ossification. The process relies on the coordinated synthesis and degradation of cartilage matrix and is disrupted in juvenile hypothyroidism, leading to abnormal skeletal development. Mast cells synthesize and store matrix-degrading enzymes. We examined whether thyroid status influences skeletal mast cell distribution in growing rats to determine whether they might modulate the actions of T(3) in bone. Tibiae were collected for histological, histochemical, immunohistochemical, and immunofluorescence analysis. Mast cells were increased throughout the bone marrow in hypothyroid rats compared with euthyroid, thyrotoxic, and hypothyroid-thyroxine replaced animals. Large numbers were present in metaphyseal marrow adjacent to the growth plate in hypothyroid animals and cells were distributed evenly throughout the marrow. Very few mast cells were present in metaphyseal marrow in other groups, but their numbers increased with increasing distance from the growth plate. T(3) receptor alpha1 (TRalpha1) was expressed in the nucleus and cytoplasm of skeletal mast cells, whereas TRalpha2 and TRbeta1 were restricted to the cytoplasm. Localization of TRs was not affected by altered thyroid status. Thus, disrupted endochondral ossification in hypothyroidism may be mediated in part by skeletal mast cells, which express TR proteins and may function as T(3) target cells.
Affiliation:
Department of Endocrinology, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow. 2002, 30 (1):259-66 Bone
Journal:
Bone
Issue Date:
Jan-2002
URI:
http://hdl.handle.net/10541/80228
PubMed ID:
11792595
Type:
Article
Language:
en
ISSN:
8756-3282
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSiebler, T-
dc.contributor.authorRobson, Helen-
dc.contributor.authorBromley, Michael-
dc.contributor.authorStevens, D A-
dc.contributor.authorShalet, Stephen M-
dc.contributor.authorWilliams, Graham R-
dc.date.accessioned2009-09-08T09:41:02Z-
dc.date.available2009-09-08T09:41:02Z-
dc.date.issued2002-01-
dc.identifier.citationThyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow. 2002, 30 (1):259-66 Boneen
dc.identifier.issn8756-3282-
dc.identifier.pmid11792595-
dc.identifier.urihttp://hdl.handle.net/10541/80228-
dc.description.abstractThyroid hormone (T(3)) plays a key role in endochondral ossification. The process relies on the coordinated synthesis and degradation of cartilage matrix and is disrupted in juvenile hypothyroidism, leading to abnormal skeletal development. Mast cells synthesize and store matrix-degrading enzymes. We examined whether thyroid status influences skeletal mast cell distribution in growing rats to determine whether they might modulate the actions of T(3) in bone. Tibiae were collected for histological, histochemical, immunohistochemical, and immunofluorescence analysis. Mast cells were increased throughout the bone marrow in hypothyroid rats compared with euthyroid, thyrotoxic, and hypothyroid-thyroxine replaced animals. Large numbers were present in metaphyseal marrow adjacent to the growth plate in hypothyroid animals and cells were distributed evenly throughout the marrow. Very few mast cells were present in metaphyseal marrow in other groups, but their numbers increased with increasing distance from the growth plate. T(3) receptor alpha1 (TRalpha1) was expressed in the nucleus and cytoplasm of skeletal mast cells, whereas TRalpha2 and TRbeta1 were restricted to the cytoplasm. Localization of TRs was not affected by altered thyroid status. Thus, disrupted endochondral ossification in hypothyroidism may be mediated in part by skeletal mast cells, which express TR proteins and may function as T(3) target cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Count-
dc.subject.meshHypothyroidism-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMale-
dc.subject.meshMast Cells-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshReceptors, Thyroid Hormone-
dc.subject.meshThyroid Hormone Receptors alpha-
dc.subject.meshThyroid Hormone Receptors beta-
dc.subject.meshThyrotoxicosis-
dc.subject.meshThyroxine-
dc.titleThyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow.en
dc.typeArticleen
dc.contributor.departmentDepartment of Endocrinology, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalBoneen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.