Association of transforming growth factor beta-1 single nucleotide polymorphisms with radiation-induced damage to normal tissues in breast cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/79116
Title:
Association of transforming growth factor beta-1 single nucleotide polymorphisms with radiation-induced damage to normal tissues in breast cancer patients.
Authors:
Quarmby, Steven L; Fakhoury, H; Levine, Edward; Barber, J; Wylie, James P; Hajeer, A H; West, Catharine M L; Stewart, Alan L; Magee, Brian; Kumar, Shant
Abstract:
PURPOSE: To investigate whether transforming growth factor beta-1 (TGFbeta1) single nucleotide polymorphisms were associated with the susceptibility of breast cancer patients to severe radiation-induced normal tissue damage. MATERIALS AND METHODS: PCR-RFLP assays were performed for TGFbeta1 gene polymorphisms on DNA obtained from 103 breast cancer patients who received radiotherapy. The G-800A, C-509T, T+869C and G+915C polymorphic sites were examined, and genotype and allele frequencies of two subgroups of patients were calculated and compared. RESULTS: The less prevalent -509T and +869C alleles were significantly associated with a subgroup of patients who developed severe radiation-induced normal tissue fibrosis (n=15) when compared with those who did not (n=88) (odds ratio=3.4, p=0.0036, and 2.37, p=0.035, respectively). Furthermore, patients with the -509TT or +869CC genotypes were between seven and 15 times more likely to develop severe fibrosis. CONCLUSIONS: These findings imply a role for the -509T and +869C alleles in the pathobiological mechanisms underlying susceptibility to radiation-induced fibrosis. Their predictive value would be limited to patients who are -509TT or +869CC, but if "fibrosis-associated" polymorphic sites in other genes could be identified, it may be possible to detect fibrosis prone individuals before radiotherapy with greater certainty.
Affiliation:
Department of Pathological Sciences, Stopford Building, Manchester University, Manchester M13 9PT, UK. steven.l.quarmby@man.ac.uk
Citation:
Association of transforming growth factor beta-1 single nucleotide polymorphisms with radiation-induced damage to normal tissues in breast cancer patients. 2003, 79 (2):137-43 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Feb-2003
URI:
http://hdl.handle.net/10541/79116
DOI:
10.1080/0955300021000045673
PubMed ID:
12569017
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorQuarmby, Steven L-
dc.contributor.authorFakhoury, H-
dc.contributor.authorLevine, Edward-
dc.contributor.authorBarber, J-
dc.contributor.authorWylie, James P-
dc.contributor.authorHajeer, A H-
dc.contributor.authorWest, Catharine M L-
dc.contributor.authorStewart, Alan L-
dc.contributor.authorMagee, Brian-
dc.contributor.authorKumar, Shant-
dc.date.accessioned2009-08-28T11:51:33Z-
dc.date.available2009-08-28T11:51:33Z-
dc.date.issued2003-02-
dc.identifier.citationAssociation of transforming growth factor beta-1 single nucleotide polymorphisms with radiation-induced damage to normal tissues in breast cancer patients. 2003, 79 (2):137-43 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid12569017-
dc.identifier.doi10.1080/0955300021000045673-
dc.identifier.urihttp://hdl.handle.net/10541/79116-
dc.description.abstractPURPOSE: To investigate whether transforming growth factor beta-1 (TGFbeta1) single nucleotide polymorphisms were associated with the susceptibility of breast cancer patients to severe radiation-induced normal tissue damage. MATERIALS AND METHODS: PCR-RFLP assays were performed for TGFbeta1 gene polymorphisms on DNA obtained from 103 breast cancer patients who received radiotherapy. The G-800A, C-509T, T+869C and G+915C polymorphic sites were examined, and genotype and allele frequencies of two subgroups of patients were calculated and compared. RESULTS: The less prevalent -509T and +869C alleles were significantly associated with a subgroup of patients who developed severe radiation-induced normal tissue fibrosis (n=15) when compared with those who did not (n=88) (odds ratio=3.4, p=0.0036, and 2.37, p=0.035, respectively). Furthermore, patients with the -509TT or +869CC genotypes were between seven and 15 times more likely to develop severe fibrosis. CONCLUSIONS: These findings imply a role for the -509T and +869C alleles in the pathobiological mechanisms underlying susceptibility to radiation-induced fibrosis. Their predictive value would be limited to patients who are -509TT or +869CC, but if "fibrosis-associated" polymorphic sites in other genes could be identified, it may be possible to detect fibrosis prone individuals before radiotherapy with greater certainty.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectDNA Canceren
dc.subject.meshAlleles-
dc.subject.meshBase Sequence-
dc.subject.meshBreast Neoplasms-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshFemale-
dc.subject.meshFibrosis-
dc.subject.meshHumans-
dc.subject.meshPolymorphism, Single Nucleotide-
dc.subject.meshRadiation Injuries-
dc.subject.meshRadiation Tolerance-
dc.subject.meshTransforming Growth Factor beta-
dc.subject.meshTransforming Growth Factor beta1-
dc.titleAssociation of transforming growth factor beta-1 single nucleotide polymorphisms with radiation-induced damage to normal tissues in breast cancer patients.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathological Sciences, Stopford Building, Manchester University, Manchester M13 9PT, UK. steven.l.quarmby@man.ac.uken
dc.identifier.journalInternational Journal of Radiation Biologyen

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