2.50
Hdl Handle:
http://hdl.handle.net/10541/79040
Title:
Electron microscopy in the study of myofibroblastic lesions.
Authors:
Eyden, Brian P
Abstract:
Electron microscopy in the diagnosis and academic study of myofibroblastic lesions is discussed. Myofibroblasts from granulation tissue and tumor stroma are regarded as the nearest equivalent to a "normal" myofibroblast population with which to define myofibroblastic differentiation in tumoral and pseudotumoral lesions. Histological features include a plump-spindle-cell morphology, with an ill-defined cytoplasm paler and less fibrillar than in smooth-muscle cells, and matrix collagen. Myofibroblasts stain for alpha-smooth-muscle actin, fibronectin, and vimentin. Desmin is found in some lesional myofibroblasts. The main ultrastructural features are prominent rough endoplasmic reticulum, modestly developed myofilaments with focal densities ("stress fibers"), and fibronexus junctions. The latter are foci on the cell surface where intracellular myofilaments and extracellular fibronectin filaments converge. Myofibroblastic lesions vary in the extent to which they mirror this overall phenotype. Hypertrophic scar, Dupuytren's disease, nodular fasciitis, the fibromatoses, and inflammatory myofibroblastic tumors have the most developed myofibroblastic features. Keloid, postoperative spindle-cell nodule, and fibroma of tendon sheath are less well differentiated. Myofibroblastoma is among many lesions described as myofibroblastic which, however, appear to show a kind of smooth-muscle differentiation. Some spindle-cell malignancies express myofibroblastic features.
Affiliation:
Department of Histopathology, Christie Hospital National Health Service Trust, Manchester, United Kingdom. brian.eyden@christie-tr.nwest.nhs.uk
Citation:
Electron microscopy in the study of myofibroblastic lesions. 2003, 20 (1):13-24 Semin Diagn Pathol
Journal:
Seminars in Diagnostic Pathology
Issue Date:
Feb-2003
URI:
http://hdl.handle.net/10541/79040
PubMed ID:
12693672
Type:
Article
Language:
en
ISSN:
0740-2570
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorEyden, Brian P-
dc.date.accessioned2009-08-28T09:17:55Z-
dc.date.available2009-08-28T09:17:55Z-
dc.date.issued2003-02-
dc.identifier.citationElectron microscopy in the study of myofibroblastic lesions. 2003, 20 (1):13-24 Semin Diagn Patholen
dc.identifier.issn0740-2570-
dc.identifier.pmid12693672-
dc.identifier.urihttp://hdl.handle.net/10541/79040-
dc.description.abstractElectron microscopy in the diagnosis and academic study of myofibroblastic lesions is discussed. Myofibroblasts from granulation tissue and tumor stroma are regarded as the nearest equivalent to a "normal" myofibroblast population with which to define myofibroblastic differentiation in tumoral and pseudotumoral lesions. Histological features include a plump-spindle-cell morphology, with an ill-defined cytoplasm paler and less fibrillar than in smooth-muscle cells, and matrix collagen. Myofibroblasts stain for alpha-smooth-muscle actin, fibronectin, and vimentin. Desmin is found in some lesional myofibroblasts. The main ultrastructural features are prominent rough endoplasmic reticulum, modestly developed myofilaments with focal densities ("stress fibers"), and fibronexus junctions. The latter are foci on the cell surface where intracellular myofilaments and extracellular fibronectin filaments converge. Myofibroblastic lesions vary in the extent to which they mirror this overall phenotype. Hypertrophic scar, Dupuytren's disease, nodular fasciitis, the fibromatoses, and inflammatory myofibroblastic tumors have the most developed myofibroblastic features. Keloid, postoperative spindle-cell nodule, and fibroma of tendon sheath are less well differentiated. Myofibroblastoma is among many lesions described as myofibroblastic which, however, appear to show a kind of smooth-muscle differentiation. Some spindle-cell malignancies express myofibroblastic features.en
dc.language.isoenen
dc.subjectCancer Proteinsen
dc.subjectMuscle Tissue Canceren
dc.subjectBiological Tumour Markersen
dc.subject.meshFibroblasts-
dc.subject.meshFibronectins-
dc.subject.meshHumans-
dc.subject.meshMicrofilaments-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMuscle Cells-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshNeoplasms, Muscle Tissue-
dc.subject.meshStromal Cells-
dc.subject.meshTumor Markers, Biological-
dc.titleElectron microscopy in the study of myofibroblastic lesions.en
dc.typeArticleen
dc.contributor.departmentDepartment of Histopathology, Christie Hospital National Health Service Trust, Manchester, United Kingdom. brian.eyden@christie-tr.nwest.nhs.uken
dc.identifier.journalSeminars in Diagnostic Pathologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.