2.50
Hdl Handle:
http://hdl.handle.net/10541/78473
Title:
X-epilectin: a novel epidermal fucolectin regulated by BMP signalling.
Authors:
Massé, Karine; Baldwin, Rebecca J; Barnett, Mark W; Jones, Elizabeth A
Abstract:
This paper reports the cloning and characterisation of a new posterior epidermal marker, X-epilectin, in Xenopus laevis. This gene encodes for a fucolectin, which belongs to the lectin superfamily of carbohydrate binding proteins and specifically binds fucose residues. RT-PCR and in situ hybridisation show that the expression of this gene is switched on during gastrulation and up-regulated during neurula stages and found expressed ubiquitously throughout the epidermis. From tailbud stages, the expression is limited to the dorsal posterior region of the embryo, suggesting that X-epilectin expression is regulated along anteroposterior and dorsoventral gradients during development. In the adult, X-epilectin is mainly expressed in intestinal components, kidney, spinal cord and skin. The effects of growth factors on the regulation of X-epilectin were studied. Change of the fate of animal caps into cement gland or dorsal mesoderm induces a down-regulation of X-epilectin expression in explants treated respectively with ammonium chloride and activin A. We also show that X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway.
Affiliation:
Molecular Physiology, Department of Biological Sciences, Warwick University, Coventry, UK.
Citation:
X-epilectin: a novel epidermal fucolectin regulated by BMP signalling. 2004, 48 (10):1119-29 Int. J. Dev. Biol.
Journal:
The International Journal of Developmental Biology
Issue Date:
Dec-2004
URI:
http://hdl.handle.net/10541/78473
DOI:
10.1387/ijdb.041892km
PubMed ID:
15602698
Type:
Article
Language:
en
ISSN:
0214-6282
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMassé, Karine-
dc.contributor.authorBaldwin, Rebecca J-
dc.contributor.authorBarnett, Mark W-
dc.contributor.authorJones, Elizabeth A-
dc.date.accessioned2009-08-25T11:27:00Z-
dc.date.available2009-08-25T11:27:00Z-
dc.date.issued2004-12-
dc.identifier.citationX-epilectin: a novel epidermal fucolectin regulated by BMP signalling. 2004, 48 (10):1119-29 Int. J. Dev. Biol.en
dc.identifier.issn0214-6282-
dc.identifier.pmid15602698-
dc.identifier.doi10.1387/ijdb.041892km-
dc.identifier.urihttp://hdl.handle.net/10541/78473-
dc.description.abstractThis paper reports the cloning and characterisation of a new posterior epidermal marker, X-epilectin, in Xenopus laevis. This gene encodes for a fucolectin, which belongs to the lectin superfamily of carbohydrate binding proteins and specifically binds fucose residues. RT-PCR and in situ hybridisation show that the expression of this gene is switched on during gastrulation and up-regulated during neurula stages and found expressed ubiquitously throughout the epidermis. From tailbud stages, the expression is limited to the dorsal posterior region of the embryo, suggesting that X-epilectin expression is regulated along anteroposterior and dorsoventral gradients during development. In the adult, X-epilectin is mainly expressed in intestinal components, kidney, spinal cord and skin. The effects of growth factors on the regulation of X-epilectin were studied. Change of the fate of animal caps into cement gland or dorsal mesoderm induces a down-regulation of X-epilectin expression in explants treated respectively with ammonium chloride and activin A. We also show that X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway.en
dc.language.isoenen
dc.subject.meshActivins-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAmmonium Chloride-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshBone Morphogenetic Protein 4-
dc.subject.meshBone Morphogenetic Protein Receptors-
dc.subject.meshBone Morphogenetic Proteins-
dc.subject.meshCarrier Proteins-
dc.subject.meshCell Differentiation-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA, Complementary-
dc.subject.meshDown-Regulation-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshIn Situ Hybridization-
dc.subject.meshInhibin-beta Subunits-
dc.subject.meshLectins-
dc.subject.meshModels, Genetic-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshProteins-
dc.subject.meshRNA-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, Growth Factor-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshSignal Transduction-
dc.subject.meshSkin-
dc.subject.meshTime Factors-
dc.subject.meshTissue Distribution-
dc.subject.meshUp-Regulation-
dc.subject.meshXenopus Proteins-
dc.subject.meshXenopus laevis-
dc.titleX-epilectin: a novel epidermal fucolectin regulated by BMP signalling.en
dc.typeArticleen
dc.contributor.departmentMolecular Physiology, Department of Biological Sciences, Warwick University, Coventry, UK.en
dc.identifier.journalThe International Journal of Developmental Biologyen

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