Tracking mesoderm formation and specification to the hemangioblast in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78440
Title:
Tracking mesoderm formation and specification to the hemangioblast in vitro.
Authors:
Lacaud, Georges; Keller, Gordon; Kouskoff, Valerie
Abstract:
During development of the mouse embryo, blood cells are generated from mesodermal precursors at specific times and locations. Using various in vivo and in vitro systems, we are now starting to understand the cascade of molecular events leading to the commitment of mesoderm and the formation of the first blood precursors, the hemangioblast. The in vitro differentiation of embryonic stem (ES) cells has proved to be an invaluable model to study lineage commitment, because one can access and easily manipulate large quantities of early progenitor cells. To help us track mesodermal subpopulations and study their specification toward blood lineages, we have engineered an ES cell line that expresses the green fluorescence protein exclusively in the mesoderm germ layer, under the control of the Brachyury regulatory sequences.
Affiliation:
Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, UK.
Citation:
Tracking mesoderm formation and specification to the hemangioblast in vitro. 2004, 14 (8):314-7 Trends Cardiovasc. Med.
Journal:
Trends in Cardiovascular Medicine
Issue Date:
Nov-2004
URI:
http://hdl.handle.net/10541/78440
DOI:
10.1016/j.tcm.2004.09.004
PubMed ID:
15596108
Type:
Article
Language:
en
ISSN:
1050-1738
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLacaud, Georges-
dc.contributor.authorKeller, Gordon-
dc.contributor.authorKouskoff, Valerie-
dc.date.accessioned2009-08-25T11:00:45Z-
dc.date.available2009-08-25T11:00:45Z-
dc.date.issued2004-11-
dc.identifier.citationTracking mesoderm formation and specification to the hemangioblast in vitro. 2004, 14 (8):314-7 Trends Cardiovasc. Med.en
dc.identifier.issn1050-1738-
dc.identifier.pmid15596108-
dc.identifier.doi10.1016/j.tcm.2004.09.004-
dc.identifier.urihttp://hdl.handle.net/10541/78440-
dc.description.abstractDuring development of the mouse embryo, blood cells are generated from mesodermal precursors at specific times and locations. Using various in vivo and in vitro systems, we are now starting to understand the cascade of molecular events leading to the commitment of mesoderm and the formation of the first blood precursors, the hemangioblast. The in vitro differentiation of embryonic stem (ES) cells has proved to be an invaluable model to study lineage commitment, because one can access and easily manipulate large quantities of early progenitor cells. To help us track mesodermal subpopulations and study their specification toward blood lineages, we have engineered an ES cell line that expresses the green fluorescence protein exclusively in the mesoderm germ layer, under the control of the Brachyury regulatory sequences.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBlood Cells-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Lineage-
dc.subject.meshEmbryonic Induction-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshGene Expression-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMesoderm-
dc.subject.meshMice-
dc.titleTracking mesoderm formation and specification to the hemangioblast in vitro.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, UK.en
dc.identifier.journalTrends in Cardiovascular Medicineen

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