Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78436
Title:
Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.
Authors:
Grallert, Agnes; Krapp, Andrea; Bagley, Steven; Simanis, Viesturs; Hagan, Iain M
Abstract:
Mitotic exit in Saccharomyces cerevisiae and septation in Schizosaccharomyces pombe are regulated by a conserved signaling network called the mitotic exit and septum initiation networks (SIN), respectively. The network is active on one of the two anaphase B spindle-pole bodies (SPBs). Whereas the inherent asymmetry of growth by budding accounts for elements of the asymmetry in S. cerevisiae, it has been unclear how, or why, the pathway is asymmetric in S. pombe. We show that elements of SPB duplication in S. pombe are conservative, and that the SIN is active on the new SPB. SIN association with the new SPB persists after transient depolymerization of microtubules. The localization of the NIMA-related kinase, Fin1, reveals further complexity in SPB inheritance. Fin1 associates with the SPB bearing the older components in all cells and with the "new" SPB in half of the population. Fin1 only binds the new SPB when this new SPB has arisen from the duplication of an SPB that is two or more cycles old. Thus, each of the four SPBs generated over two consecutive cell cycles are different, because they have distinct fates in the next cell cycle. Fin1 binds the SPB once the SIN is active and the association requires the SIN inhibitors Byr4 and Cdc16. Fin1 physically associates with Byr4. Compromising Fin1 function leads to SIN activation on both anaphase B SPBs and promotes septation, indicating that Fin1 restrains SIN activity on the old SPB.
Affiliation:
Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, Manchester M20 4BX, UK.
Citation:
Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. 2004, 18 (9):1007-21 Genes Dev.
Journal:
Genes & Development
Issue Date:
1-May-2004
URI:
http://hdl.handle.net/10541/78436
DOI:
10.1101/gad.296204
PubMed ID:
15132994
Type:
Article
Language:
en
ISSN:
0890-9369
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGrallert, Agnes-
dc.contributor.authorKrapp, Andrea-
dc.contributor.authorBagley, Steven-
dc.contributor.authorSimanis, Viesturs-
dc.contributor.authorHagan, Iain M-
dc.date.accessioned2009-08-25T10:57:24Z-
dc.date.available2009-08-25T10:57:24Z-
dc.date.issued2004-05-01-
dc.identifier.citationRecruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. 2004, 18 (9):1007-21 Genes Dev.en
dc.identifier.issn0890-9369-
dc.identifier.pmid15132994-
dc.identifier.doi10.1101/gad.296204-
dc.identifier.urihttp://hdl.handle.net/10541/78436-
dc.description.abstractMitotic exit in Saccharomyces cerevisiae and septation in Schizosaccharomyces pombe are regulated by a conserved signaling network called the mitotic exit and septum initiation networks (SIN), respectively. The network is active on one of the two anaphase B spindle-pole bodies (SPBs). Whereas the inherent asymmetry of growth by budding accounts for elements of the asymmetry in S. cerevisiae, it has been unclear how, or why, the pathway is asymmetric in S. pombe. We show that elements of SPB duplication in S. pombe are conservative, and that the SIN is active on the new SPB. SIN association with the new SPB persists after transient depolymerization of microtubules. The localization of the NIMA-related kinase, Fin1, reveals further complexity in SPB inheritance. Fin1 associates with the SPB bearing the older components in all cells and with the "new" SPB in half of the population. Fin1 only binds the new SPB when this new SPB has arisen from the duplication of an SPB that is two or more cycles old. Thus, each of the four SPBs generated over two consecutive cell cycles are different, because they have distinct fates in the next cell cycle. Fin1 binds the SPB once the SIN is active and the association requires the SIN inhibitors Byr4 and Cdc16. Fin1 physically associates with Byr4. Compromising Fin1 function leads to SIN activation on both anaphase B SPBs and promotes septation, indicating that Fin1 restrains SIN activity on the old SPB.en
dc.language.isoenen
dc.subject.meshCell Cycle-
dc.subject.meshCentrioles-
dc.subject.meshGenes, Fungal-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshModels, Biological-
dc.subject.meshMutation-
dc.subject.meshSchizosaccharomyces-
dc.titleRecruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Cell Division Group, Paterson Institute for Cancer Research, Manchester M20 4BX, UK.en
dc.identifier.journalGenes & Developmenten
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