Translocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78434
Title:
Translocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination.
Authors:
Fenton, James A L; Pratt, Guy; Rothwell, Dominic G; Rawstron, Andy C; Morgan, Gareth J
Abstract:
We describe the characterization of the genomic DNA breakpoints of two multiple myeloma (MM) patients with t(11;14). IGH translocation events are present in many MM tumors, and it is proposed that they occur early in the pathogenesis, based on the assumption that the translocations are simple reciprocal events mediated by errors in class-switch recombination (CSR). We provide evidence from two patients that the translocation event can be more complex, with DNA from chromosome band 11q13 joined to apparently already recombined hybrid (Smu/Sgamma) switch region sequences. In one case, there was also evidence that a further rearrangement had occurred at the t(11;14) recombination site, resulting in an inversion of 40 bp of the 5'Smu flanking sequence. This suggests that primary IGH arrangements in MM may be more complex than previous myeloma models have suggested, but that they essentially occur through illegitimate CSR events.
Affiliation:
Academic Unit of Oncology and Haematology, University of Leeds, Leeds, United Kingdom. jamesf@lrf.leeds.ac.uk
Citation:
Translocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination. 2004, 39 (2):151-5 Genes Chromosomes Cancer
Journal:
Genes, Chromosomes & Cancer
Issue Date:
Feb-2004
URI:
http://hdl.handle.net/10541/78434
DOI:
10.1002/gcc.10304
PubMed ID:
14695995
Type:
Article
Language:
en
ISSN:
1045-2257
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFenton, James A L-
dc.contributor.authorPratt, Guy-
dc.contributor.authorRothwell, Dominic G-
dc.contributor.authorRawstron, Andy C-
dc.contributor.authorMorgan, Gareth J-
dc.date.accessioned2009-08-25T10:54:34Z-
dc.date.available2009-08-25T10:54:34Z-
dc.date.issued2004-02-
dc.identifier.citationTranslocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination. 2004, 39 (2):151-5 Genes Chromosomes Canceren
dc.identifier.issn1045-2257-
dc.identifier.pmid14695995-
dc.identifier.doi10.1002/gcc.10304-
dc.identifier.urihttp://hdl.handle.net/10541/78434-
dc.description.abstractWe describe the characterization of the genomic DNA breakpoints of two multiple myeloma (MM) patients with t(11;14). IGH translocation events are present in many MM tumors, and it is proposed that they occur early in the pathogenesis, based on the assumption that the translocations are simple reciprocal events mediated by errors in class-switch recombination (CSR). We provide evidence from two patients that the translocation event can be more complex, with DNA from chromosome band 11q13 joined to apparently already recombined hybrid (Smu/Sgamma) switch region sequences. In one case, there was also evidence that a further rearrangement had occurred at the t(11;14) recombination site, resulting in an inversion of 40 bp of the 5'Smu flanking sequence. This suggests that primary IGH arrangements in MM may be more complex than previous myeloma models have suggested, but that they essentially occur through illegitimate CSR events.en
dc.language.isoenen
dc.subject.meshBase Sequence-
dc.subject.meshChromosome Aberrations-
dc.subject.meshChromosome Breakage-
dc.subject.meshChromosome Mapping-
dc.subject.meshChromosomes, Human, Pair 11-
dc.subject.meshChromosomes, Human, Pair 14-
dc.subject.meshHumans-
dc.subject.meshModels, Genetic-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMultiple Myeloma-
dc.subject.meshRecombination, Genetic-
dc.subject.meshTranslocation, Genetic-
dc.titleTranslocation t(11;14) in multiple myeloma: Analysis of translocation breakpoints on der(11) and der(14) chromosomes suggests complex molecular mechanisms of recombination.en
dc.typeArticleen
dc.contributor.departmentAcademic Unit of Oncology and Haematology, University of Leeds, Leeds, United Kingdom. jamesf@lrf.leeds.ac.uken
dc.identifier.journalGenes, Chromosomes & Canceren

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