Haemangioblast commitment is initiated in the primitive streak of the mouse embryo.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78377
Title:
Haemangioblast commitment is initiated in the primitive streak of the mouse embryo.
Authors:
Huber, Tara L; Kouskoff, Valerie; Fehling, H Joerg; Palis, James; Keller, Gordon
Abstract:
Haematopoietic and vascular cells are thought to arise from a common progenitor called the haemangioblast. Support for this concept has been provided by embryonic stem (ES) cell differentiation studies that identified the blast colony-forming cell (BL-CFC), a progenitor with both haematopoietic and vascular potential. Using conditions that support the growth of BL-CFCs, we identify comparable progenitors that can form blast cell colonies (displaying haematopoietic and vascular potential) in gastrulating mouse embryos. Cell mixing and limiting dilution analyses provide evidence that these colonies are clonal, indicating that they develop from a progenitor with haemangioblast potential. Embryo-derived haemangioblasts are first detected at the mid-streak stage of gastrulation and peak in number during the neural plate stage. Analysis of embryos carrying complementary DNA of the green fluorescent protein targeted to the brachyury locus demonstrates that the haemangioblast is a subpopulation of mesoderm that co-expresses brachyury (also known as T) and Flk-1 (also known as Kdr). Detailed mapping studies reveal that haemangioblasts are found at highest frequency in the posterior region of the primitive streak, indicating that initial stages of haematopoietic and vascular commitment occur before blood island development in the yolk sac.
Affiliation:
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA.
Citation:
Haemangioblast commitment is initiated in the primitive streak of the mouse embryo. 2004, 432 (7017):625-30 Nature
Journal:
Nature
Issue Date:
2-Dec-2004
URI:
http://hdl.handle.net/10541/78377
DOI:
10.1038/nature03122
PubMed ID:
15577911
Type:
Article
Language:
en
ISSN:
1476-4687
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHuber, Tara L-
dc.contributor.authorKouskoff, Valerie-
dc.contributor.authorFehling, H Joerg-
dc.contributor.authorPalis, James-
dc.contributor.authorKeller, Gordon-
dc.date.accessioned2009-08-24T14:14:18Z-
dc.date.available2009-08-24T14:14:18Z-
dc.date.issued2004-12-02-
dc.identifier.citationHaemangioblast commitment is initiated in the primitive streak of the mouse embryo. 2004, 432 (7017):625-30 Natureen
dc.identifier.issn1476-4687-
dc.identifier.pmid15577911-
dc.identifier.doi10.1038/nature03122-
dc.identifier.urihttp://hdl.handle.net/10541/78377-
dc.description.abstractHaematopoietic and vascular cells are thought to arise from a common progenitor called the haemangioblast. Support for this concept has been provided by embryonic stem (ES) cell differentiation studies that identified the blast colony-forming cell (BL-CFC), a progenitor with both haematopoietic and vascular potential. Using conditions that support the growth of BL-CFCs, we identify comparable progenitors that can form blast cell colonies (displaying haematopoietic and vascular potential) in gastrulating mouse embryos. Cell mixing and limiting dilution analyses provide evidence that these colonies are clonal, indicating that they develop from a progenitor with haemangioblast potential. Embryo-derived haemangioblasts are first detected at the mid-streak stage of gastrulation and peak in number during the neural plate stage. Analysis of embryos carrying complementary DNA of the green fluorescent protein targeted to the brachyury locus demonstrates that the haemangioblast is a subpopulation of mesoderm that co-expresses brachyury (also known as T) and Flk-1 (also known as Kdr). Detailed mapping studies reveal that haemangioblasts are found at highest frequency in the posterior region of the primitive streak, indicating that initial stages of haematopoietic and vascular commitment occur before blood island development in the yolk sac.en
dc.language.isoenen
dc.subjectHaemangioblastomaen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Lineage-
dc.subject.meshClone Cells-
dc.subject.meshEmbryo, Mammalian-
dc.subject.meshFemale-
dc.subject.meshGastrula-
dc.subject.meshHemangioblastoma-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMale-
dc.subject.meshMesoderm-
dc.subject.meshMice-
dc.subject.meshYolk Sac-
dc.titleHaemangioblast commitment is initiated in the primitive streak of the mouse embryo.en
dc.typeArticleen
dc.contributor.departmentDepartment of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA.en
dc.identifier.journalNatureen

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