Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78368
Title:
Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.
Authors:
Wilkinson, Caroline R M; Dittmar, Gunnar A G; Ohi, Melanie D; Uetz, Peter; Jones, Nic; Finley, Daniel
Abstract:
Hub1/Ubl5 is a member of the family of ubiquitin-like proteins (UBLs). The tertiary structure of Hub1 is similar to that of ubiquitin; however, it differs from known modifiers in that there is no conserved glycine residue near the C terminus which, in ubiquitin and UBLs, is required for covalent modification of target proteins. Instead, there is a conserved dityrosine motif proximal to the terminal nonconserved amino acid. In S. cerevisiae, high molecular weight adducts can be formed in vivo from Hub1, but the structure of these adducts is not known, and they could be either covalent or noncovalent. The budding yeast HUB1 gene is not essential, but Delta hub1 mutants display defects in mating. Here, we report that fission yeast hub1 is an essential gene, whose loss results in cell cycle defects and inefficient pre-mRNA splicing. A screen for Hub1 interactors identified Snu66, a component of the U4/U6.U5 tri-snRNP splicing complex. Furthermore, overexpression of Snu66 suppresses the lethality of a hub1ts mutant. In cells lacking functional hub1, the nuclear localization of Snu66 is disrupted, suggesting that an important role for Hub1 is the correct subcellular targeting of Snu66, although our data suggest that Hub1 is likely to perform other roles in splicing as well.
Affiliation:
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. cwilkinson@picr.man.ac.uk
Citation:
Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast. 2004, 14 (24):2283-8 Curr. Biol.
Journal:
Current Biology
Issue Date:
29-Dec-2004
URI:
http://hdl.handle.net/10541/78368
DOI:
10.1016/j.cub.2004.11.058
PubMed ID:
15620657
Type:
Article
Language:
en
ISSN:
0960-9822
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilkinson, Caroline R M-
dc.contributor.authorDittmar, Gunnar A G-
dc.contributor.authorOhi, Melanie D-
dc.contributor.authorUetz, Peter-
dc.contributor.authorJones, Nic-
dc.contributor.authorFinley, Daniel-
dc.date.accessioned2009-08-24T16:17:39Z-
dc.date.available2009-08-24T16:17:39Z-
dc.date.issued2004-12-29-
dc.identifier.citationUbiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast. 2004, 14 (24):2283-8 Curr. Biol.en
dc.identifier.issn0960-9822-
dc.identifier.pmid15620657-
dc.identifier.doi10.1016/j.cub.2004.11.058-
dc.identifier.urihttp://hdl.handle.net/10541/78368-
dc.description.abstractHub1/Ubl5 is a member of the family of ubiquitin-like proteins (UBLs). The tertiary structure of Hub1 is similar to that of ubiquitin; however, it differs from known modifiers in that there is no conserved glycine residue near the C terminus which, in ubiquitin and UBLs, is required for covalent modification of target proteins. Instead, there is a conserved dityrosine motif proximal to the terminal nonconserved amino acid. In S. cerevisiae, high molecular weight adducts can be formed in vivo from Hub1, but the structure of these adducts is not known, and they could be either covalent or noncovalent. The budding yeast HUB1 gene is not essential, but Delta hub1 mutants display defects in mating. Here, we report that fission yeast hub1 is an essential gene, whose loss results in cell cycle defects and inefficient pre-mRNA splicing. A screen for Hub1 interactors identified Snu66, a component of the U4/U6.U5 tri-snRNP splicing complex. Furthermore, overexpression of Snu66 suppresses the lethality of a hub1ts mutant. In cells lacking functional hub1, the nuclear localization of Snu66 is disrupted, suggesting that an important role for Hub1 is the correct subcellular targeting of Snu66, although our data suggest that Hub1 is likely to perform other roles in splicing as well.en
dc.language.isoenen
dc.subject.meshCell Cycle-
dc.subject.meshElectrophoresis, Polyacrylamide Gel-
dc.subject.meshFlow Cytometry-
dc.subject.meshGenes, Essential-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshMutation-
dc.subject.meshOligonucleotides-
dc.subject.meshProtein Transport-
dc.subject.meshRNA Splicing-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshRibonucleoproteins, Small Nuclear-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.subject.meshTwo-Hybrid System Techniques-
dc.subject.meshUbiquitin-Protein Ligase Complexes-
dc.titleUbiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.en
dc.typeArticleen
dc.contributor.departmentDepartment of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. cwilkinson@picr.man.ac.uken
dc.identifier.journalCurrent Biologyen

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