Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78367
Title:
Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions.
Authors:
Ward, Christopher M; Barrow, Katie M; Stern, Peter L
Abstract:
Mouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated this further by comparing the differentiation characteristics of five independently derived ES cell lines cultured and differentiated under defined conditions. Undifferentiated ES cell lines exhibited similar morphology and antigen/transcript marker expression. However, upon differentiation in monolayer culture by LIF withdrawal, only two of the lines expressed similar germ layer transcript profiles, and these were significantly altered compared to differentiation in serum-supplemented media. Neurofilament-68k was the only transcript marker common to all cell lines, however, induction of neuroectoderm lineages using 1 microM all-trans retinoic acid (RA) resulted in significant variations in cell number and morphology between the lines. Furthermore, neurons were only formed from clones of the two cell lines that exhibited similar transcript profiles, although the morphology was different between the two. We conclude that the independent ES cell lines in this study differ in their response to alterations in culture conditions in vitro, and the use of an appropriate cell line enables relatively homogeneous neuronal populations to be achieved in monolayer culture under defined conditions.
Affiliation:
Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. wardcm@hotmail.com
Citation:
Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions. 2004, 293 (2):229-38 Exp. Cell Res.
Journal:
Experimental Cell Research
Issue Date:
15-Feb-2004
URI:
http://hdl.handle.net/10541/78367
DOI:
10.1016/j.yexcr.2003.10.017
PubMed ID:
14729460
Language:
en
ISSN:
0014-4827
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWard, Christopher M-
dc.contributor.authorBarrow, Katie M-
dc.contributor.authorStern, Peter L-
dc.date.accessioned2009-08-24T16:13:39Z-
dc.date.available2009-08-24T16:13:39Z-
dc.date.issued2004-02-15-
dc.identifier.citationSignificant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions. 2004, 293 (2):229-38 Exp. Cell Res.en
dc.identifier.issn0014-4827-
dc.identifier.pmid14729460-
dc.identifier.doi10.1016/j.yexcr.2003.10.017-
dc.identifier.urihttp://hdl.handle.net/10541/78367-
dc.description.abstractMouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated this further by comparing the differentiation characteristics of five independently derived ES cell lines cultured and differentiated under defined conditions. Undifferentiated ES cell lines exhibited similar morphology and antigen/transcript marker expression. However, upon differentiation in monolayer culture by LIF withdrawal, only two of the lines expressed similar germ layer transcript profiles, and these were significantly altered compared to differentiation in serum-supplemented media. Neurofilament-68k was the only transcript marker common to all cell lines, however, induction of neuroectoderm lineages using 1 microM all-trans retinoic acid (RA) resulted in significant variations in cell number and morphology between the lines. Furthermore, neurons were only formed from clones of the two cell lines that exhibited similar transcript profiles, although the morphology was different between the two. We conclude that the independent ES cell lines in this study differ in their response to alterations in culture conditions in vitro, and the use of an appropriate cell line enables relatively homogeneous neuronal populations to be achieved in monolayer culture under defined conditions.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBiological Markers-
dc.subject.meshCell Count-
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshCell Lineage-
dc.subject.meshCell Size-
dc.subject.meshClone Cells-
dc.subject.meshCulture Media-
dc.subject.meshGenetic Variation-
dc.subject.meshMice-
dc.subject.meshNeurofilament Proteins-
dc.subject.meshPluripotent Stem Cells-
dc.subject.meshTranscription, Genetic-
dc.subject.meshTretinoin-
dc.titleSignificant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions.en
dc.contributor.departmentImmunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK. wardcm@hotmail.comen
dc.identifier.journalExperimental Cell Researchen

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