Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78229
Title:
Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas.
Authors:
Nordsmark, Marianne; Loncaster, Juliette A; Aquino-Parsons, Christina; Chou, Shu-Chuan; Ladekarl, Morten; Havsteen, Hanne; Lindegaard, Jacob C; Davidson, Susan E; Varia, Mahesh; West, Catharine M L; Hunter, Robin D; Overgaard, Jens; Raleigh, James A
Abstract:
BACKGROUND AND PURPOSE: The measurement of tumour oxygenation using Eppendorf oxygen-sensitive needle electrodes can provide prognostic information but the method is limited to accessible tumours that are suitable for electrode insertion. In this paper the aim was to study the relationship between such physiological measurements of tumour hypoxia and the labelling of tumours with the hypoxia-specific marker pimonidazole. MATERIALS AND METHODS: Assessment of tumour oxygen partial pressure (pO(2)) using an Eppendorf pO(2) histograph and immunohistochemical pimonidazole labelling was carried out in 86 patients with primary cervix carcinomas. Pimonidazole was given as a single injection (0.5 g/m(2) i.v.) and 10-24 h later pO(2) measurements were made and biopsies taken. Tumour oxygenation status was evaluated as the median tumour pO(2) and the fraction of pO(2) values </=10 mmHg (HP(10)), </=5 mmHg (HP(5)) and </=2.5 mmHg (HP(2.5)). Hypoxia was detected by immunohistochemistry using monoclonal antibodies directed against reductively activated pimonidazole. Pimonidazole binding was scored using a light microscope. Each tumour was evaluated by the relative area pimonidazole at highest score and the accumulated area of pimonidazole labelling from score 1 to 4. Necrosis was measured in HE stained sections. RESULTS AND CONCLUSIONS: The degree of hypoxia assessed by either pimonidazole binding or invasive electrode measurements varied significantly between tumours. There was a trend that the most hypoxic tumours measured by oxygen electrodes had the highest score of necrosis, and no or little pimonidazole binding. However, this observation was not consistent and there was no correlation between pimonidazole staining expressed in this way and oxygen electrode measurements of hypoxia.
Affiliation:
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark.
Citation:
Measurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas. 2003, 67 (1):35-44 Radiother Oncol
Journal:
Radiotherapy and Oncology
Issue Date:
Apr-2003
URI:
http://hdl.handle.net/10541/78229
PubMed ID:
12758238
Type:
Article
Language:
en
ISSN:
0167-8140
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorNordsmark, Marianne-
dc.contributor.authorLoncaster, Juliette A-
dc.contributor.authorAquino-Parsons, Christina-
dc.contributor.authorChou, Shu-Chuan-
dc.contributor.authorLadekarl, Morten-
dc.contributor.authorHavsteen, Hanne-
dc.contributor.authorLindegaard, Jacob C-
dc.contributor.authorDavidson, Susan E-
dc.contributor.authorVaria, Mahesh-
dc.contributor.authorWest, Catharine M L-
dc.contributor.authorHunter, Robin D-
dc.contributor.authorOvergaard, Jens-
dc.contributor.authorRaleigh, James A-
dc.date.accessioned2009-08-21T14:35:06Z-
dc.date.available2009-08-21T14:35:06Z-
dc.date.issued2003-04-
dc.identifier.citationMeasurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas. 2003, 67 (1):35-44 Radiother Oncolen
dc.identifier.issn0167-8140-
dc.identifier.pmid12758238-
dc.identifier.urihttp://hdl.handle.net/10541/78229-
dc.description.abstractBACKGROUND AND PURPOSE: The measurement of tumour oxygenation using Eppendorf oxygen-sensitive needle electrodes can provide prognostic information but the method is limited to accessible tumours that are suitable for electrode insertion. In this paper the aim was to study the relationship between such physiological measurements of tumour hypoxia and the labelling of tumours with the hypoxia-specific marker pimonidazole. MATERIALS AND METHODS: Assessment of tumour oxygen partial pressure (pO(2)) using an Eppendorf pO(2) histograph and immunohistochemical pimonidazole labelling was carried out in 86 patients with primary cervix carcinomas. Pimonidazole was given as a single injection (0.5 g/m(2) i.v.) and 10-24 h later pO(2) measurements were made and biopsies taken. Tumour oxygenation status was evaluated as the median tumour pO(2) and the fraction of pO(2) values </=10 mmHg (HP(10)), </=5 mmHg (HP(5)) and </=2.5 mmHg (HP(2.5)). Hypoxia was detected by immunohistochemistry using monoclonal antibodies directed against reductively activated pimonidazole. Pimonidazole binding was scored using a light microscope. Each tumour was evaluated by the relative area pimonidazole at highest score and the accumulated area of pimonidazole labelling from score 1 to 4. Necrosis was measured in HE stained sections. RESULTS AND CONCLUSIONS: The degree of hypoxia assessed by either pimonidazole binding or invasive electrode measurements varied significantly between tumours. There was a trend that the most hypoxic tumours measured by oxygen electrodes had the highest score of necrosis, and no or little pimonidazole binding. However, this observation was not consistent and there was no correlation between pimonidazole staining expressed in this way and oxygen electrode measurements of hypoxia.en
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAnalysis of Variance-
dc.subject.meshCarcinoma-
dc.subject.meshCell Hypoxia-
dc.subject.meshComputer Graphics-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMicroelectrodes-
dc.subject.meshNitroimidazoles-
dc.subject.meshOxygen-
dc.subject.meshPolarography-
dc.subject.meshRadiation-Sensitizing Agents-
dc.subject.meshRetrospective Studies-
dc.subject.meshStatistics, Nonparametric-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleMeasurements of hypoxia using pimonidazole and polarographic oxygen-sensitive electrodes in human cervix carcinomas.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark.en
dc.identifier.journalRadiotherapy and Oncologyen

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