Haploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells.

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Hdl Handle:
http://hdl.handle.net/10541/78142
Title:
Haploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells.
Authors:
Lacaud, Georges; Kouskoff, Valerie; Trumble, Anne; Schwantz, Staci; Keller, Gordon
Abstract:
The AML1 gene (recently renamed Runx1), which encodes the DNA-binding subunit of a transcription factor of the core binding factor (CBF) family, is required for the establishment of definitive hematopoiesis. We have previously demonstrated that Runx1 is expressed in yolk sac mesodermal cells prior to the establishment of the blood islands and in the embryoid body (EB)-derived blast-colony-forming cells (BL-CFCs), the in vitro equivalent of the hemangioblast. Analysis of Runx1-deficient embryonic stem (ES) cells demonstrated that this gene is essential for the generation of normal numbers of blast colonies, the progeny of the BL-CFCs. In the present study, we analyzed the potential of Runx1(+/-) ES cells to determine if heterozygosity at the Runx1 locus impacts early developmental events leading to the commitment of the BL-CFCs. Our results indicate that Runx1 heterozygosity leads to an acceleration of mesodermal commitment and specification to the BL-CFCs and to the hematopoietic lineages in EBs.
Affiliation:
Carl C. Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
Citation:
Haploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells. 2004, 103 (3):886-9 Blood
Journal:
Blood
Issue Date:
1-Feb-2004
URI:
http://hdl.handle.net/10541/78142
DOI:
10.1182/blood-2003-06-2149
PubMed ID:
14525762
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLacaud, Georges-
dc.contributor.authorKouskoff, Valerie-
dc.contributor.authorTrumble, Anne-
dc.contributor.authorSchwantz, Staci-
dc.contributor.authorKeller, Gordon-
dc.date.accessioned2009-08-21T11:04:48Z-
dc.date.available2009-08-21T11:04:48Z-
dc.date.issued2004-02-01-
dc.identifier.citationHaploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells. 2004, 103 (3):886-9 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid14525762-
dc.identifier.doi10.1182/blood-2003-06-2149-
dc.identifier.urihttp://hdl.handle.net/10541/78142-
dc.description.abstractThe AML1 gene (recently renamed Runx1), which encodes the DNA-binding subunit of a transcription factor of the core binding factor (CBF) family, is required for the establishment of definitive hematopoiesis. We have previously demonstrated that Runx1 is expressed in yolk sac mesodermal cells prior to the establishment of the blood islands and in the embryoid body (EB)-derived blast-colony-forming cells (BL-CFCs), the in vitro equivalent of the hemangioblast. Analysis of Runx1-deficient embryonic stem (ES) cells demonstrated that this gene is essential for the generation of normal numbers of blast colonies, the progeny of the BL-CFCs. In the present study, we analyzed the potential of Runx1(+/-) ES cells to determine if heterozygosity at the Runx1 locus impacts early developmental events leading to the commitment of the BL-CFCs. Our results indicate that Runx1 heterozygosity leads to an acceleration of mesodermal commitment and specification to the BL-CFCs and to the hematopoietic lineages in EBs.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCore Binding Factor Alpha 2 Subunit-
dc.subject.meshDNA, Complementary-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshEmbryo, Mammalian-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMesoderm-
dc.subject.meshMice-
dc.subject.meshMice, Knockout-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshTranscription Factors-
dc.titleHaploinsufficiency of Runx1 results in the acceleration of mesodermal development and hemangioblast specification upon in vitro differentiation of ES cells.en
dc.typeArticleen
dc.contributor.departmentCarl C. Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.en
dc.identifier.journalBlooden

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