Amplification protocols introduce systematic but reproducible errors into gene expression studies.

2.50
Hdl Handle:
http://hdl.handle.net/10541/78132
Title:
Amplification protocols introduce systematic but reproducible errors into gene expression studies.
Authors:
Wilson, Claire L; Pepper, Stuart D; Hey, Yvonne; Miller, Crispin J
Abstract:
The desire to perform microarray experiments with small starting amounts of RNA has led to the development of a variety of protocols for preparing and amplifying mRNA. This has consequences not only for the standardization of experimental design, but also for reproducibility and comparability between experiments. Here we investigate the differences between the Affymetrix standard and small sample protocols and address the data analysis issues that arise when comparing samples and experiments that have been processed in different ways. We show that data generated on the same platform using different protocols are not directly comparable. Further, protocols introduce systematic biases that can be largely accounted for by using the correct data analysis techniques.
Affiliation:
Paterson Institute for Cancer Research, Withington, Manchester, UK.
Citation:
Amplification protocols introduce systematic but reproducible errors into gene expression studies. 2004, 36 (3):498-506 BioTechniques
Journal:
BioTechniques
Issue Date:
Mar-2004
URI:
http://hdl.handle.net/10541/78132
PubMed ID:
15038166
Type:
Article
Language:
en
ISSN:
0736-6205
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilson, Claire L-
dc.contributor.authorPepper, Stuart D-
dc.contributor.authorHey, Yvonne-
dc.contributor.authorMiller, Crispin J-
dc.date.accessioned2009-08-21T10:22:06Z-
dc.date.available2009-08-21T10:22:06Z-
dc.date.issued2004-03-
dc.identifier.citationAmplification protocols introduce systematic but reproducible errors into gene expression studies. 2004, 36 (3):498-506 BioTechniquesen
dc.identifier.issn0736-6205-
dc.identifier.pmid15038166-
dc.identifier.urihttp://hdl.handle.net/10541/78132-
dc.description.abstractThe desire to perform microarray experiments with small starting amounts of RNA has led to the development of a variety of protocols for preparing and amplifying mRNA. This has consequences not only for the standardization of experimental design, but also for reproducibility and comparability between experiments. Here we investigate the differences between the Affymetrix standard and small sample protocols and address the data analysis issues that arise when comparing samples and experiments that have been processed in different ways. We show that data generated on the same platform using different protocols are not directly comparable. Further, protocols introduce systematic biases that can be largely accounted for by using the correct data analysis techniques.en
dc.language.isoenen
dc.subject.meshData Interpretation, Statistical-
dc.subject.meshGene Expression Profiling-
dc.subject.meshNucleic Acid Amplification Techniques-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshQuality Control-
dc.subject.meshRNA-
dc.subject.meshReproducibility of Results-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshSpecimen Handling-
dc.titleAmplification protocols introduce systematic but reproducible errors into gene expression studies.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Withington, Manchester, UK.en
dc.identifier.journalBioTechniquesen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.