Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.

2.50
Hdl Handle:
http://hdl.handle.net/10541/77753
Title:
Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.
Authors:
Guo, Baoqiang; Rooney, Paul; Slevin, Mark; Li, Chenggang; Parameshwar, Sudeep; Liu, Donghui; Kumar, Patricia; Bernabeu, Carmelo; Kumar, Shant
Abstract:
CD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.
Affiliation:
Laboratory Medicine Academic Group, The Medical School, University of Manchester, Manchester, M13 9PT, UK. baoqiang.guo@man.ac.uk
Citation:
Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival. 2004, 25 (2):285-91 Int. J. Oncol.
Journal:
International Journal of Oncology
Issue Date:
Aug-2004
URI:
http://hdl.handle.net/10541/77753
PubMed ID:
15254724
Type:
Article
Language:
en
ISSN:
1019-6439
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorGuo, Baoqiang-
dc.contributor.authorRooney, Paul-
dc.contributor.authorSlevin, Mark-
dc.contributor.authorLi, Chenggang-
dc.contributor.authorParameshwar, Sudeep-
dc.contributor.authorLiu, Donghui-
dc.contributor.authorKumar, Patricia-
dc.contributor.authorBernabeu, Carmelo-
dc.contributor.authorKumar, Shant-
dc.date.accessioned2009-08-18T16:01:45Z-
dc.date.available2009-08-18T16:01:45Z-
dc.date.issued2004-08-
dc.identifier.citationOverexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival. 2004, 25 (2):285-91 Int. J. Oncol.en
dc.identifier.issn1019-6439-
dc.identifier.pmid15254724-
dc.identifier.urihttp://hdl.handle.net/10541/77753-
dc.description.abstractCD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD29-
dc.subject.meshCell Adhesion-
dc.subject.meshCell Survival-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshMitogen-Activated Protein Kinase 8-
dc.subject.meshMyoblasts-
dc.subject.meshPhosphorylation-
dc.subject.meshRats-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshSignal Transduction-
dc.subject.meshTransfection-
dc.subject.meshUp-Regulation-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.titleOverexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.en
dc.typeArticleen
dc.contributor.departmentLaboratory Medicine Academic Group, The Medical School, University of Manchester, Manchester, M13 9PT, UK. baoqiang.guo@man.ac.uken
dc.identifier.journalInternational Journal of Oncologyen

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