Side population/ABCG2-positive cells represent a heterogeneous group of haemopoietic cells: implications for the use of adult stem cells in transplantation and plasticity protocols.

2.50
Hdl Handle:
http://hdl.handle.net/10541/76857
Title:
Side population/ABCG2-positive cells represent a heterogeneous group of haemopoietic cells: implications for the use of adult stem cells in transplantation and plasticity protocols.
Authors:
Naylor, C S; Jaworska, Ewa; Branson, Kate; Embleton, Jim; Chopra, Rajesh
Abstract:
Murine side population (SP) cells may have an increased ability to engraft lethally irradiated mice and lack CD34 expression. Strategies using CD34 as a primary marker of haemopoietic stem cells may therefore result in the exclusion of a primitive stem cell population. The molecular basis for the murine SP phenotype has been attributed to the multidrug-resistance transporter ABCG2. This study aimed to investigate ABCG2 expression from a variety of human sources and investigate the relationship between ABCG2 expression, the SP phenotype, and expression of markers such as CD34 and CD133. SP cells were observed in different haemopoietic sources, but a significant increase in the number of SP cells was observed in PB following granulocyte colony-stimulating factor mobilisation. No direct correlation between the frequency of SP cells and the expression of ABCG2 was observed. SP cells were identified in both lineage-positive and lineage-negative population and ABCG2 expression was enriched in lineage-negative SP cells. Lineage-negative SP cells were devoid of CD34 expression but enriched for CD133. Subsequent analysis revealed that ABCG2 and CD133 are coexpressed. Together, these data suggest that the ABCG2 transporter is neither required nor responsible for the SP phenotpye in many human blood cells.
Affiliation:
Stem Cell and Leukamia Biology Group, and Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Side population/ABCG2-positive cells represent a heterogeneous group of haemopoietic cells: implications for the use of adult stem cells in transplantation and plasticity protocols. 2005, 35 (4):353-60 Bone Marrow Transplant.
Journal:
Bone Marrow Transplantation
Issue Date:
Feb-2005
URI:
http://hdl.handle.net/10541/76857
DOI:
10.1038/sj.bmt.1704762
PubMed ID:
15608658
Type:
Article
Language:
en
ISSN:
0268-3369
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorNaylor, C S-
dc.contributor.authorJaworska, Ewa-
dc.contributor.authorBranson, Kate-
dc.contributor.authorEmbleton, Jim-
dc.contributor.authorChopra, Rajesh-
dc.date.accessioned2009-08-10T17:30:32Z-
dc.date.available2009-08-10T17:30:32Z-
dc.date.issued2005-02-
dc.identifier.citationSide population/ABCG2-positive cells represent a heterogeneous group of haemopoietic cells: implications for the use of adult stem cells in transplantation and plasticity protocols. 2005, 35 (4):353-60 Bone Marrow Transplant.en
dc.identifier.issn0268-3369-
dc.identifier.pmid15608658-
dc.identifier.doi10.1038/sj.bmt.1704762-
dc.identifier.urihttp://hdl.handle.net/10541/76857-
dc.description.abstractMurine side population (SP) cells may have an increased ability to engraft lethally irradiated mice and lack CD34 expression. Strategies using CD34 as a primary marker of haemopoietic stem cells may therefore result in the exclusion of a primitive stem cell population. The molecular basis for the murine SP phenotype has been attributed to the multidrug-resistance transporter ABCG2. This study aimed to investigate ABCG2 expression from a variety of human sources and investigate the relationship between ABCG2 expression, the SP phenotype, and expression of markers such as CD34 and CD133. SP cells were observed in different haemopoietic sources, but a significant increase in the number of SP cells was observed in PB following granulocyte colony-stimulating factor mobilisation. No direct correlation between the frequency of SP cells and the expression of ABCG2 was observed. SP cells were identified in both lineage-positive and lineage-negative population and ABCG2 expression was enriched in lineage-negative SP cells. Lineage-negative SP cells were devoid of CD34 expression but enriched for CD133. Subsequent analysis revealed that ABCG2 and CD133 are coexpressed. Together, these data suggest that the ABCG2 transporter is neither required nor responsible for the SP phenotpye in many human blood cells.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subjectCancer Proteinsen
dc.subject.meshATP-Binding Cassette Transporters-
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD34-
dc.subject.meshCell Lineage-
dc.subject.meshFetal Blood-
dc.subject.meshGlycoproteins-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshPeptides-
dc.subject.meshPhenotype-
dc.subject.meshStem Cell Transplantation-
dc.titleSide population/ABCG2-positive cells represent a heterogeneous group of haemopoietic cells: implications for the use of adult stem cells in transplantation and plasticity protocols.en
dc.typeArticleen
dc.contributor.departmentStem Cell and Leukamia Biology Group, and Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBone Marrow Transplantationen

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