Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124.

2.50
Hdl Handle:
http://hdl.handle.net/10541/76825
Title:
Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124.
Authors:
Dekker, Bronwen A; Keen, Heather G; Shaw, David M; Disley, Lynn; Hastings, David L; Hadfield, John A; Reader, Andrew J; Allan, Donald; Julyan, Peter J; Watson, Alastair; Zweit, Jamal
Abstract:
We are interested in imaging cell death in vivo using annexin V radiolabeled with (124)I. In this study, [(124)I]4IB-annexin V and [(124)I]4IB-ovalbumin were made using [(124)I]N-hydroxysuccinimidyl-4-iodobenzoate prepared by iododestannylation of N-hydroxysuccinimidyl-4-(tributylstannyl)benzoate. [(124)I]4IB-annexin V binds to phosphatidylserine-coated microtiter plates and apoptotic Jurkat cells and accumulates in hepatic apoptotic lesions in mice treated with anti-Fas antibody, while [(124)I]4IB-ovalbumin does not. In comparison with (124)I-annexin V, [(124)I]4IB-annexin V has a higher rate of binding to phosphatidylserine in vitro, a higher kidney and urine uptake, a lower thyroid and stomach content uptake, greater plasma stability and a lower rate of plasma clearance. Binding of radioactivity to apoptotic cells relative to normal cells in vitro and in vivo appears to be lower for [(124)I]4IB-annexin V than for (124)I-annexin V.
Affiliation:
CRUK/UMIST Department of Radiochemical Targeting and Imaging, Paterson Institute for Cancer Research, M20 4BX Manchester, UK. bdekker@picr.man.ac.uk
Citation:
Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124. 2005, 32 (4):403-13 Nucl. Med. Biol.
Journal:
Nuclear Medicine and Biology
Issue Date:
May-2005
URI:
http://hdl.handle.net/10541/76825
DOI:
10.1016/j.nucmedbio.2005.02.002
PubMed ID:
15878510
Type:
Article
Language:
en
ISSN:
0969-8051
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDekker, Bronwen A-
dc.contributor.authorKeen, Heather G-
dc.contributor.authorShaw, David M-
dc.contributor.authorDisley, Lynn-
dc.contributor.authorHastings, David L-
dc.contributor.authorHadfield, John A-
dc.contributor.authorReader, Andrew J-
dc.contributor.authorAllan, Donald-
dc.contributor.authorJulyan, Peter J-
dc.contributor.authorWatson, Alastair-
dc.contributor.authorZweit, Jamal-
dc.date.accessioned2009-08-10T16:44:59Z-
dc.date.available2009-08-10T16:44:59Z-
dc.date.issued2005-05-
dc.identifier.citationFunctional comparison of annexin V analogues labeled indirectly and directly with iodine-124. 2005, 32 (4):403-13 Nucl. Med. Biol.en
dc.identifier.issn0969-8051-
dc.identifier.pmid15878510-
dc.identifier.doi10.1016/j.nucmedbio.2005.02.002-
dc.identifier.urihttp://hdl.handle.net/10541/76825-
dc.description.abstractWe are interested in imaging cell death in vivo using annexin V radiolabeled with (124)I. In this study, [(124)I]4IB-annexin V and [(124)I]4IB-ovalbumin were made using [(124)I]N-hydroxysuccinimidyl-4-iodobenzoate prepared by iododestannylation of N-hydroxysuccinimidyl-4-(tributylstannyl)benzoate. [(124)I]4IB-annexin V binds to phosphatidylserine-coated microtiter plates and apoptotic Jurkat cells and accumulates in hepatic apoptotic lesions in mice treated with anti-Fas antibody, while [(124)I]4IB-ovalbumin does not. In comparison with (124)I-annexin V, [(124)I]4IB-annexin V has a higher rate of binding to phosphatidylserine in vitro, a higher kidney and urine uptake, a lower thyroid and stomach content uptake, greater plasma stability and a lower rate of plasma clearance. Binding of radioactivity to apoptotic cells relative to normal cells in vitro and in vivo appears to be lower for [(124)I]4IB-annexin V than for (124)I-annexin V.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAnnexin A5-
dc.subject.meshApoptosis-
dc.subject.meshHumans-
dc.subject.meshIodine Radioisotopes-
dc.subject.meshJurkat Cells-
dc.subject.meshMetabolic Clearance Rate-
dc.subject.meshMice-
dc.subject.meshOrgan Specificity-
dc.subject.meshRadiopharmaceuticals-
dc.subject.meshTissue Distribution-
dc.titleFunctional comparison of annexin V analogues labeled indirectly and directly with iodine-124.en
dc.typeArticleen
dc.contributor.departmentCRUK/UMIST Department of Radiochemical Targeting and Imaging, Paterson Institute for Cancer Research, M20 4BX Manchester, UK. bdekker@picr.man.ac.uken
dc.identifier.journalNuclear Medicine and Biologyen

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