A cross-linker-sensitive myeloid leukemia cell line from a 2-year-old boy with severe Fanconi anemia and biallelic FANCD1/BRCA2 mutations.

2.50
Hdl Handle:
http://hdl.handle.net/10541/76808
Title:
A cross-linker-sensitive myeloid leukemia cell line from a 2-year-old boy with severe Fanconi anemia and biallelic FANCD1/BRCA2 mutations.
Authors:
Meyer, Stefan; Fergusson, William D; Oostra, Anneke B; Medhurst, Annette L; Waisfisz, Quinten; De Winter, Johan P; Chen, Fei; Carr, Trevor F; Clayton-Smith, Jill; Clancy, Tara; Green, Michael; Barber, Lisa M; Eden, Tim O B; Will, Andrew M; Joenje, Hans; Taylor, G Malcolm
Abstract:
Fanconi anemia (FA) is a rare autosomal recessive disorder characterized by congenital and developmental abnormalities, hypersensitivity to DNA cross-linking agents such as mitomycin C (MMC), and strong predisposition to acute myeloid leukemia (AML). In this article, we describe clinical and molecular findings in a boy with a severe FA phenotype who developed AML by the age of 2. Although he lacked a strong family history of cancer, he was subsequently shown to carry biallelic mutations in the FANCD1/BRCA2 gene. These included an IVS7 splice-site mutation, which is strongly associated with early AML in homozygous or compound heterozygous carrier status in FA-D1 patients. Myeloid leukemia cells from this patient have been maintained in culture for more than 1 year and have been designated as the SB1690CB cell line. Growth of SB1690CB is dependent on granulocyte macrophage colony stimulating factor or interleukin-3. This cell line has retained its MMC sensitivity and has undergone further spontaneous changes in the spectrum of cytogenetic aberrations compared with the primary leukemia. This is the second AML cell line derived from an FA-D1 patient and the first proof that malignant clones arising in FA patients can retain inherited MMC sensitivity. As FA-derived malignancies are normally not very responsive to treatment, this implies there are important mechanisms of acquiring correction of the cellular FA phenotype that would explain the poor chemoresponsiveness observed in FA-associated malignancies and might also play a role in the initiation and progression of cancer in the general population.
Affiliation:
Cancer-Immunogenetics Laboratory, University of Manchester, St. Mary's Hospital, Hathersage Road, Manchester M13 0JH, United Kingdom. stefan.meyer@man.ac.uk
Citation:
A cross-linker-sensitive myeloid leukemia cell line from a 2-year-old boy with severe Fanconi anemia and biallelic FANCD1/BRCA2 mutations. 2005, 42 (4):404-15 Genes Chromosomes Cancer
Journal:
Genes, Chromosomes & Cancer
Issue Date:
Apr-2005
URI:
http://hdl.handle.net/10541/76808
DOI:
10.1002/gcc.20153
PubMed ID:
15645491
Type:
Article
Language:
en
ISSN:
1045-2257
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorMeyer, Stefan-
dc.contributor.authorFergusson, William D-
dc.contributor.authorOostra, Anneke B-
dc.contributor.authorMedhurst, Annette L-
dc.contributor.authorWaisfisz, Quinten-
dc.contributor.authorDe Winter, Johan P-
dc.contributor.authorChen, Fei-
dc.contributor.authorCarr, Trevor F-
dc.contributor.authorClayton-Smith, Jill-
dc.contributor.authorClancy, Tara-
dc.contributor.authorGreen, Michael-
dc.contributor.authorBarber, Lisa M-
dc.contributor.authorEden, Tim O B-
dc.contributor.authorWill, Andrew M-
dc.contributor.authorJoenje, Hans-
dc.contributor.authorTaylor, G Malcolm-
dc.date.accessioned2009-08-10T10:49:35Z-
dc.date.available2009-08-10T10:49:35Z-
dc.date.issued2005-04-
dc.identifier.citationA cross-linker-sensitive myeloid leukemia cell line from a 2-year-old boy with severe Fanconi anemia and biallelic FANCD1/BRCA2 mutations. 2005, 42 (4):404-15 Genes Chromosomes Canceren
dc.identifier.issn1045-2257-
dc.identifier.pmid15645491-
dc.identifier.doi10.1002/gcc.20153-
dc.identifier.urihttp://hdl.handle.net/10541/76808-
dc.description.abstractFanconi anemia (FA) is a rare autosomal recessive disorder characterized by congenital and developmental abnormalities, hypersensitivity to DNA cross-linking agents such as mitomycin C (MMC), and strong predisposition to acute myeloid leukemia (AML). In this article, we describe clinical and molecular findings in a boy with a severe FA phenotype who developed AML by the age of 2. Although he lacked a strong family history of cancer, he was subsequently shown to carry biallelic mutations in the FANCD1/BRCA2 gene. These included an IVS7 splice-site mutation, which is strongly associated with early AML in homozygous or compound heterozygous carrier status in FA-D1 patients. Myeloid leukemia cells from this patient have been maintained in culture for more than 1 year and have been designated as the SB1690CB cell line. Growth of SB1690CB is dependent on granulocyte macrophage colony stimulating factor or interleukin-3. This cell line has retained its MMC sensitivity and has undergone further spontaneous changes in the spectrum of cytogenetic aberrations compared with the primary leukemia. This is the second AML cell line derived from an FA-D1 patient and the first proof that malignant clones arising in FA patients can retain inherited MMC sensitivity. As FA-derived malignancies are normally not very responsive to treatment, this implies there are important mechanisms of acquiring correction of the cellular FA phenotype that would explain the poor chemoresponsiveness observed in FA-associated malignancies and might also play a role in the initiation and progression of cancer in the general population.en
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectLeukaemiaen
dc.subject.meshAlleles-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCell Division-
dc.subject.meshCell Line, Tumor-
dc.subject.meshChild, Preschool-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshFanconi Anemia Complementation Group Proteins-
dc.subject.meshGenes, BRCA2-
dc.subject.meshHumans-
dc.subject.meshImmunophenotyping-
dc.subject.meshKaryotyping-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshMale-
dc.subject.meshMitomycin-
dc.subject.meshMutation-
dc.subject.meshNuclear Proteins-
dc.titleA cross-linker-sensitive myeloid leukemia cell line from a 2-year-old boy with severe Fanconi anemia and biallelic FANCD1/BRCA2 mutations.en
dc.typeArticleen
dc.contributor.departmentCancer-Immunogenetics Laboratory, University of Manchester, St. Mary's Hospital, Hathersage Road, Manchester M13 0JH, United Kingdom. stefan.meyer@man.ac.uken
dc.identifier.journalGenes, Chromosomes & Canceren

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