Segregation of genomes in polyploid tumour cells following mitotic catastrophe.

2.50
Hdl Handle:
http://hdl.handle.net/10541/76277
Title:
Segregation of genomes in polyploid tumour cells following mitotic catastrophe.
Authors:
Erenpreisa, Jekaterina; Kalejs, Martins; Ianzini, Fiorenza; Kosmacek, Elizabeth A; Mackey, Mike; Emzinsh, Dzintars; Cragg, Mark S; Ivanov, Andrei; Illidge, Timothy M ( 0000-0003-3191-7324 )
Abstract:
Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.
Affiliation:
Biomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia. katrina@biomed.lu.lv
Citation:
Segregation of genomes in polyploid tumour cells following mitotic catastrophe. 2005, 29 (12):1005-11 Cell Biol. Int.
Journal:
Cell Biology International
Issue Date:
Dec-2005
URI:
http://hdl.handle.net/10541/76277
DOI:
10.1016/j.cellbi.2005.10.008
PubMed ID:
16314119
Type:
Article
Language:
en
ISSN:
1065-6995
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorErenpreisa, Jekaterina-
dc.contributor.authorKalejs, Martins-
dc.contributor.authorIanzini, Fiorenza-
dc.contributor.authorKosmacek, Elizabeth A-
dc.contributor.authorMackey, Mike-
dc.contributor.authorEmzinsh, Dzintars-
dc.contributor.authorCragg, Mark S-
dc.contributor.authorIvanov, Andrei-
dc.contributor.authorIllidge, Timothy M-
dc.date.accessioned2009-08-04T17:17:11Z-
dc.date.available2009-08-04T17:17:11Z-
dc.date.issued2005-12-
dc.identifier.citationSegregation of genomes in polyploid tumour cells following mitotic catastrophe. 2005, 29 (12):1005-11 Cell Biol. Int.en
dc.identifier.issn1065-6995-
dc.identifier.pmid16314119-
dc.identifier.doi10.1016/j.cellbi.2005.10.008-
dc.identifier.urihttp://hdl.handle.net/10541/76277-
dc.description.abstractFollowing irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.en
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshCell Line, Tumor-
dc.subject.meshChromosome Segregation-
dc.subject.meshHela Cells-
dc.subject.meshHumans-
dc.subject.meshJurkat Cells-
dc.subject.meshMetaphase-
dc.subject.meshMitosis-
dc.subject.meshPolyploidy-
dc.subject.meshTumor Suppressor Protein p53-
dc.titleSegregation of genomes in polyploid tumour cells following mitotic catastrophe.en
dc.typeArticleen
dc.contributor.departmentBiomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia. katrina@biomed.lu.lven
dc.identifier.journalCell Biology Internationalen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.