Estimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections.

2.50
Hdl Handle:
http://hdl.handle.net/10541/74919
Title:
Estimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections.
Authors:
Greenfield, Hayley M; Sweeney, D A; Newton, R K; Leather, Angela; Murray, John; Angelica, R; Swindell, Ric; Chang, James
Abstract:
Peripheral blood stem cells are increasingly used to ensure rapid haematological engraftment after myeloablative chemotherapy. After mobilization, progenitor cells in the blood can be enumerated to predict an adequate collection by leukapheresis. The Advia 120 automated counter has an immature cell channel measuring a parameter known as large undifferentiated cells (LUC's), which were quantified to assess their value in refining the timing of apheresis. Data were available from 102 apheresis sessions. Positive correlation was found for peripheral blood CD34+ cells and apheresis counts (r = 0.82, P < 0.0005) but not for total WCC (r = -0.15, P = 0.13) or LUC count (r = 0.12, P = 0.23). Our results indicate that the LUC population in peripheral blood has no relevance to the subsequent CD34 content of the apheresis product and CD34 cell enumeration by flow cytometry is advocated.
Affiliation:
Department of Haematology, Christie Hospital, Manchester, UK. hayleygreenfield@tiscali.co.uk
Citation:
Estimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections. 2005, 27 (5):287-91 Clin Lab Haematol
Journal:
Clinical and Laboratory Haematology
Issue Date:
Oct-2005
URI:
http://hdl.handle.net/10541/74919
DOI:
10.1111/j.1365-2257.2005.00712.x
PubMed ID:
16178906
Type:
Article
Language:
en
ISSN:
0141-9854
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorGreenfield, Hayley M-
dc.contributor.authorSweeney, D A-
dc.contributor.authorNewton, R K-
dc.contributor.authorLeather, Angela-
dc.contributor.authorMurray, John-
dc.contributor.authorAngelica, R-
dc.contributor.authorSwindell, Ric-
dc.contributor.authorChang, James-
dc.date.accessioned2009-07-22T10:19:29Z-
dc.date.available2009-07-22T10:19:29Z-
dc.date.issued2005-10-
dc.identifier.citationEstimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections. 2005, 27 (5):287-91 Clin Lab Haematolen
dc.identifier.issn0141-9854-
dc.identifier.pmid16178906-
dc.identifier.doi10.1111/j.1365-2257.2005.00712.x-
dc.identifier.urihttp://hdl.handle.net/10541/74919-
dc.description.abstractPeripheral blood stem cells are increasingly used to ensure rapid haematological engraftment after myeloablative chemotherapy. After mobilization, progenitor cells in the blood can be enumerated to predict an adequate collection by leukapheresis. The Advia 120 automated counter has an immature cell channel measuring a parameter known as large undifferentiated cells (LUC's), which were quantified to assess their value in refining the timing of apheresis. Data were available from 102 apheresis sessions. Positive correlation was found for peripheral blood CD34+ cells and apheresis counts (r = 0.82, P < 0.0005) but not for total WCC (r = -0.15, P = 0.13) or LUC count (r = 0.12, P = 0.23). Our results indicate that the LUC population in peripheral blood has no relevance to the subsequent CD34 content of the apheresis product and CD34 cell enumeration by flow cytometry is advocated.en
dc.language.isoenen
dc.subjectHaematologic Diseasesen
dc.subjectHaematopoietic Stem Cell Mobilisationen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34-
dc.subject.meshArea Under Curve-
dc.subject.meshBlood Component Removal-
dc.subject.meshCell Count-
dc.subject.meshFlow Cytometry-
dc.subject.meshHematologic Diseases-
dc.subject.meshHematopoietic Stem Cell Mobilization-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshLeukocyte Count-
dc.subject.meshPractice Guidelines as Topic-
dc.titleEstimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections.en
dc.typeArticleen
dc.contributor.departmentDepartment of Haematology, Christie Hospital, Manchester, UK. hayleygreenfield@tiscali.co.uken
dc.identifier.journalClinical and Laboratory Haematologyen

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