Metastatic small cell malignant melanoma: a case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes.

2.50
Hdl Handle:
http://hdl.handle.net/10541/74874
Title:
Metastatic small cell malignant melanoma: a case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes.
Authors:
Eyden, Brian P; Moss, Jill; Shore, Ian; Banerjee, Saumitra S
Abstract:
A case of metastatic malignant melanoma exhibiting small cell morphology is described. The patient had had a previous primary nodular small cell melanoma. The metastatic tumor was examined by conventional histology, light microscope immunohistochemistry, conventional electron microscopy, and ultrastructural immunolabeling. It consisted of small cells, which, however, varied in size and were present in distinct but merging areas. Tumor cells were negative for S-100 protein and very focally positive for cytokeratin: these findings in combination with small cell morphology suggested the possibility of small cell carcinoma. However, other melanocytic markers were positive. Neuroendocrine markers were negative. By electron microscopy, tumor cells lacked unambiguous melanosomes but contained paranuclear aggregates of nondescript granules. Following ultrastructural immunolabeling, these were found to be decorated with gold-labeled HMB-45 antibodies, thereby confirming them as lattice-deficient melanosomes. This tumor is an uncommon example of malignant melanoma where immunoultrastructural analysis helped clarify the nature of otherwise nondescript granules as true but lattice-deficient melanosomes. This is also the first case of small cell melanoma to be studied by electron microscopy.
Affiliation:
Department of Histopathology, Christie Hospital NHS Trust, Manchester, UK. brian.eyden@christie-tr.nwest.nhs.uk
Citation:
Metastatic small cell malignant melanoma: a case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes., 29 (1):71-8 Ultrastruct Pathol
Journal:
Ultrastructural Pathology
Issue Date:
2005
URI:
http://hdl.handle.net/10541/74874
PubMed ID:
15931781
Type:
Article
Language:
en
ISSN:
0191-3123
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorEyden, Brian P-
dc.contributor.authorMoss, Jill-
dc.contributor.authorShore, Ian-
dc.contributor.authorBanerjee, Saumitra S-
dc.date.accessioned2009-07-22T08:42:17Z-
dc.date.available2009-07-22T08:42:17Z-
dc.date.issued2005-
dc.identifier.citationMetastatic small cell malignant melanoma: a case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes., 29 (1):71-8 Ultrastruct Patholen
dc.identifier.issn0191-3123-
dc.identifier.pmid15931781-
dc.identifier.urihttp://hdl.handle.net/10541/74874-
dc.description.abstractA case of metastatic malignant melanoma exhibiting small cell morphology is described. The patient had had a previous primary nodular small cell melanoma. The metastatic tumor was examined by conventional histology, light microscope immunohistochemistry, conventional electron microscopy, and ultrastructural immunolabeling. It consisted of small cells, which, however, varied in size and were present in distinct but merging areas. Tumor cells were negative for S-100 protein and very focally positive for cytokeratin: these findings in combination with small cell morphology suggested the possibility of small cell carcinoma. However, other melanocytic markers were positive. Neuroendocrine markers were negative. By electron microscopy, tumor cells lacked unambiguous melanosomes but contained paranuclear aggregates of nondescript granules. Following ultrastructural immunolabeling, these were found to be decorated with gold-labeled HMB-45 antibodies, thereby confirming them as lattice-deficient melanosomes. This tumor is an uncommon example of malignant melanoma where immunoultrastructural analysis helped clarify the nature of otherwise nondescript granules as true but lattice-deficient melanosomes. This is also the first case of small cell melanoma to be studied by electron microscopy.en
dc.language.isoenen
dc.subjectCancer Recurrenceen
dc.subjectSkin Canceren
dc.subjectMultiple Primary Cancersen
dc.subject.meshAged-
dc.subject.meshCarcinoma, Basal Cell-
dc.subject.meshDiagnosis, Differential-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshKeratins-
dc.subject.meshLymphatic Metastasis-
dc.subject.meshMale-
dc.subject.meshMelanoma-
dc.subject.meshMelanosomes-
dc.subject.meshMicroscopy, Electron, Transmission-
dc.subject.meshMicroscopy, Immunoelectron-
dc.subject.meshNeoplasm Recurrence, Local-
dc.subject.meshNeoplasms, Multiple Primary-
dc.subject.meshS100 Proteins-
dc.subject.meshSkin Neoplasms-
dc.titleMetastatic small cell malignant melanoma: a case requiring immunoelectronmicroscopy for the demonstration of lattice-deficient melanosomes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Histopathology, Christie Hospital NHS Trust, Manchester, UK. brian.eyden@christie-tr.nwest.nhs.uken
dc.identifier.journalUltrastructural Pathologyen
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