2.50
Hdl Handle:
http://hdl.handle.net/10541/74824
Title:
Kin4 kinase delays mitotic exit in response to spindle alignment defects.
Authors:
Pereira, Gislene; Schiebel, Elmar
Abstract:
For many polarized cells, it is critical that the mitotic spindle becomes positioned relative to the polarity axis. This is especially important in yeast, where the site of cytokinesis is predetermined. The spindle position checkpoint (SPOC) therefore delays mitotic exit of cells with a mispositioned spindle. One component of the SPOC is the Bub2-Bfa1 complex, an inhibitor of the mitotic exit network (MEN). Here, we show that the Kin4 kinase is a component of the SPOC and as such is essential to delay cell cycle progression of cells with a misaligned spindle. When spindles are correctly oriented, Kin4 and Bub2-Bfa1 are asymmetrically localized to opposite spindle pole bodies (SPBs). Bub2-Bfa1 then becomes inhibited by Cdc5 polo kinase with anaphase onset, a prerequisite for mitotic exit. In response to spindle misalignment, Kin4 and Bub2-Bfa1 are brought together at both SPBs. Kin4 now maintains Bub2-Bfa1 activity by counteracting Cdc5, thereby inhibiting mitotic exit.
Affiliation:
Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK. gislene.pereira@manchester.ac.uk
Citation:
Kin4 kinase delays mitotic exit in response to spindle alignment defects. 2005, 19 (2):209-21 Mol. Cell
Journal:
Molecular Cell
Issue Date:
22-Jul-2005
URI:
http://hdl.handle.net/10541/74824
DOI:
10.1016/j.molcel.2005.05.030
PubMed ID:
16039590
Type:
Article
Language:
en
ISSN:
1097-2765
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPereira, Gislene-
dc.contributor.authorSchiebel, Elmar-
dc.date.accessioned2009-07-21T17:01:33Z-
dc.date.available2009-07-21T17:01:33Z-
dc.date.issued2005-07-22-
dc.identifier.citationKin4 kinase delays mitotic exit in response to spindle alignment defects. 2005, 19 (2):209-21 Mol. Cellen
dc.identifier.issn1097-2765-
dc.identifier.pmid16039590-
dc.identifier.doi10.1016/j.molcel.2005.05.030-
dc.identifier.urihttp://hdl.handle.net/10541/74824-
dc.description.abstractFor many polarized cells, it is critical that the mitotic spindle becomes positioned relative to the polarity axis. This is especially important in yeast, where the site of cytokinesis is predetermined. The spindle position checkpoint (SPOC) therefore delays mitotic exit of cells with a mispositioned spindle. One component of the SPOC is the Bub2-Bfa1 complex, an inhibitor of the mitotic exit network (MEN). Here, we show that the Kin4 kinase is a component of the SPOC and as such is essential to delay cell cycle progression of cells with a misaligned spindle. When spindles are correctly oriented, Kin4 and Bub2-Bfa1 are asymmetrically localized to opposite spindle pole bodies (SPBs). Bub2-Bfa1 then becomes inhibited by Cdc5 polo kinase with anaphase onset, a prerequisite for mitotic exit. In response to spindle misalignment, Kin4 and Bub2-Bfa1 are brought together at both SPBs. Kin4 now maintains Bub2-Bfa1 activity by counteracting Cdc5, thereby inhibiting mitotic exit.en
dc.language.isoenen
dc.subject.meshCell Cycle-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCytoskeletal Proteins-
dc.subject.meshMicrotubules-
dc.subject.meshMitosis-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshProtein Kinases-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.titleKin4 kinase delays mitotic exit in response to spindle alignment defects.en
dc.typeArticleen
dc.contributor.departmentFaculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK. gislene.pereira@manchester.ac.uken
dc.identifier.journalMolecular Cellen

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