The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.

2.50
Hdl Handle:
http://hdl.handle.net/10541/73117
Title:
The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.
Authors:
Araki, Yasuhiro; Lau, Corine K; Maekawa, Hiromi; Jaspersen, Sue L; Giddings, Thomas H; Schiebel, Elmar; Winey, Mark
Abstract:
The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.
Affiliation:
The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom.
Citation:
The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. 2006, 17 (4):1959-70 Mol. Biol. Cell
Journal:
Molecular Biology of the Cell
Issue Date:
Apr-2006
URI:
http://hdl.handle.net/10541/73117
DOI:
10.1091/mbc.E05-07-0668
PubMed ID:
16436507
Type:
Article
Language:
en
ISSN:
1059-1524
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAraki, Yasuhiro-
dc.contributor.authorLau, Corine K-
dc.contributor.authorMaekawa, Hiromi-
dc.contributor.authorJaspersen, Sue L-
dc.contributor.authorGiddings, Thomas H-
dc.contributor.authorSchiebel, Elmar-
dc.contributor.authorWiney, Mark-
dc.date.accessioned2009-07-09T12:22:50Z-
dc.date.available2009-07-09T12:22:50Z-
dc.date.issued2006-04-
dc.identifier.citationThe Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. 2006, 17 (4):1959-70 Mol. Biol. Cellen
dc.identifier.issn1059-1524-
dc.identifier.pmid16436507-
dc.identifier.doi10.1091/mbc.E05-07-0668-
dc.identifier.urihttp://hdl.handle.net/10541/73117-
dc.description.abstractThe spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.en
dc.language.isoenen
dc.subject.meshAlleles-
dc.subject.meshCarrier Proteins-
dc.subject.meshCell Cycle-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCell Membrane-
dc.subject.meshCytoskeletal Proteins-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshGenes, Essential-
dc.subject.meshGenes, Fungal-
dc.subject.meshMembrane Proteins-
dc.subject.meshMicrotubule Proteins-
dc.subject.meshMicrotubules-
dc.subject.meshMitosis-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshNuclear Envelope-
dc.subject.meshNuclear Proteins-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshTwo-Hybrid System Techniques-
dc.titleThe Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom.en
dc.identifier.journalMolecular Biology of the Cellen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.