A specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72868
Title:
A specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei.
Authors:
Bibi, Rufzan; Pranesh, Nagarajan; Saunders, Mark P; Wilson, Malcolm S; O'Dwyer, Sarah T; Stern, Peter L; Renehan, Andrew G
Abstract:
Pseudomyxoma peritonei (PMP) is a rare neoplasm of mainly appendiceal origin, characterised by excess intra-abdominal mucin production leading to high morbidity and mortality. While histological features are frequently indolent, this tumour disseminates aggressively throughout the abdominal cavity, yet seldom metastasises. This study determined the expression of several markers of colorectal differentiation (carcinoembryonic antigen (CEA), cytokeratins (CK20 and CK7), epithelial membrane antigen), mucin production (MUC-2, interleukin-9 (IL-9), IL-9 receptor (IL-9Ralpha)), and cell adhesion (N- and E-cadherin, vimentin) in PMP tissue (n=26) compared with expressions in normal colonic mucosa (n=19) and colorectal adenocarcinoma (n=26). Expressions of CEA and cytokeratins were similar for PMP as those in colorectal adenocarcinomas with the exception that the CK20-/CK7- pattern was rare in PMP (Fisher's exact test: P=0.001). Similarly, expressions of mucin-related proteins were comparable for adenocarcinoma and PMP, with the exception that IL-9 expression was uncommon in adenocarcinoma (P=0.009). Pseudomyxoma peritonei demonstrated a specific pattern of adhesion-related protein expressions of increased N-cadherin, reduced E-cadherin, and increased vimentin (P=0.004), a phenotype suggesting a possible epithelial-mesenchymal transition state. Primary PMP cell cultures were successfully maintained and demonstrated marker expressions similar to those seen in in vivo tissues. These early characterisation studies demonstrate similarities between PMP and colorectal adenocarcinoma, but also reveal a specific cadherin phenotype that may characterise the distinct non-metastasising behaviour of PMP, and form the basis for future mechanistic and therapy-targeting research.
Affiliation:
Cancer Research UK Immunology Group, Paterson Institute for Cancer Research, Manchester, UK.
Citation:
A specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei. 2006, 95 (9):1258-64 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
6-Nov-2006
URI:
http://hdl.handle.net/10541/72868
DOI:
10.1038/sj.bjc.6603398
PubMed ID:
17031402
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBibi, Rufzan-
dc.contributor.authorPranesh, Nagarajan-
dc.contributor.authorSaunders, Mark P-
dc.contributor.authorWilson, Malcolm S-
dc.contributor.authorO'Dwyer, Sarah T-
dc.contributor.authorStern, Peter L-
dc.contributor.authorRenehan, Andrew G-
dc.date.accessioned2009-07-07T15:53:18Z-
dc.date.available2009-07-07T15:53:18Z-
dc.date.issued2006-11-06-
dc.identifier.citationA specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei. 2006, 95 (9):1258-64 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid17031402-
dc.identifier.doi10.1038/sj.bjc.6603398-
dc.identifier.urihttp://hdl.handle.net/10541/72868-
dc.description.abstractPseudomyxoma peritonei (PMP) is a rare neoplasm of mainly appendiceal origin, characterised by excess intra-abdominal mucin production leading to high morbidity and mortality. While histological features are frequently indolent, this tumour disseminates aggressively throughout the abdominal cavity, yet seldom metastasises. This study determined the expression of several markers of colorectal differentiation (carcinoembryonic antigen (CEA), cytokeratins (CK20 and CK7), epithelial membrane antigen), mucin production (MUC-2, interleukin-9 (IL-9), IL-9 receptor (IL-9Ralpha)), and cell adhesion (N- and E-cadherin, vimentin) in PMP tissue (n=26) compared with expressions in normal colonic mucosa (n=19) and colorectal adenocarcinoma (n=26). Expressions of CEA and cytokeratins were similar for PMP as those in colorectal adenocarcinomas with the exception that the CK20-/CK7- pattern was rare in PMP (Fisher's exact test: P=0.001). Similarly, expressions of mucin-related proteins were comparable for adenocarcinoma and PMP, with the exception that IL-9 expression was uncommon in adenocarcinoma (P=0.009). Pseudomyxoma peritonei demonstrated a specific pattern of adhesion-related protein expressions of increased N-cadherin, reduced E-cadherin, and increased vimentin (P=0.004), a phenotype suggesting a possible epithelial-mesenchymal transition state. Primary PMP cell cultures were successfully maintained and demonstrated marker expressions similar to those seen in in vivo tissues. These early characterisation studies demonstrate similarities between PMP and colorectal adenocarcinoma, but also reveal a specific cadherin phenotype that may characterise the distinct non-metastasising behaviour of PMP, and form the basis for future mechanistic and therapy-targeting research.en
dc.language.isoenen
dc.subjectCancer Metastasisen
dc.subjectPeritoneal Canceren
dc.subjectTumour Cellsen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshCadherins-
dc.subject.meshCarcinoembryonic Antigen-
dc.subject.meshDiagnosis, Differential-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshInterleukin-9-
dc.subject.meshKeratin-20-
dc.subject.meshKeratin-7-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMucin-1-
dc.subject.meshMucin-2-
dc.subject.meshMucins-
dc.subject.meshNeoplasm Metastasis-
dc.subject.meshPeritoneal Neoplasms-
dc.subject.meshPseudomyxoma Peritonei-
dc.subject.meshReceptors, Interleukin-9-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshVimentin-
dc.titleA specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Immunology Group, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.