No evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72864
Title:
No evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia.
Authors:
Meyer, Stefan; White, Daniel J; Will, Andrew M; Eden, Tim O B; Sim, Alyson; Brown, Robert; Strathdee, Gordon
Abstract:
Fanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) carry a high risk of haematological cancer. Affected cellular pathways may be modulated in sporadic malignancies and silencing of FANCF through methylation has been shown to cause somatic disruption of the FA pathway. Combined bisulphite restriction analysis for methylation of FANCF, FANCB and NBS1 was used to investigate 81 sporadic acute childhood leukaemias. No methylation was detected at any associated CpG sites analysed. This does not exclude very low levels of FANCF, FANCB or NBS1 methylation, but suggests other factors are responsible for chemo-sensitivity and chromosomal instability in sporadic childhood leukaemia.
Affiliation:
Department of Paediatric Haematology and Oncology, Central Manchester and Manchester Children's University Hospitals NHS Trust, Manchester, UK. stefan.meyer-2@manchester.ac.uk
Citation:
No evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia. 2006, 134 (1):61-3 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Jul-2006
URI:
http://hdl.handle.net/10541/72864
DOI:
10.1111/j.1365-2141.2006.06107.x
PubMed ID:
16803569
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMeyer, Stefan-
dc.contributor.authorWhite, Daniel J-
dc.contributor.authorWill, Andrew M-
dc.contributor.authorEden, Tim O B-
dc.contributor.authorSim, Alyson-
dc.contributor.authorBrown, Robert-
dc.contributor.authorStrathdee, Gordon-
dc.date.accessioned2009-07-07T15:47:38Z-
dc.date.available2009-07-07T15:47:38Z-
dc.date.issued2006-07-
dc.identifier.citationNo evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia. 2006, 134 (1):61-3 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid16803569-
dc.identifier.doi10.1111/j.1365-2141.2006.06107.x-
dc.identifier.urihttp://hdl.handle.net/10541/72864-
dc.description.abstractFanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) carry a high risk of haematological cancer. Affected cellular pathways may be modulated in sporadic malignancies and silencing of FANCF through methylation has been shown to cause somatic disruption of the FA pathway. Combined bisulphite restriction analysis for methylation of FANCF, FANCB and NBS1 was used to investigate 81 sporadic acute childhood leukaemias. No methylation was detected at any associated CpG sites analysed. This does not exclude very low levels of FANCF, FANCB or NBS1 methylation, but suggests other factors are responsible for chemo-sensitivity and chromosomal instability in sporadic childhood leukaemia.en
dc.language.isoenen
dc.subjectCancer Genesen
dc.subjectLeukaemiaen
dc.subjectLeukaemia, Myeloiden
dc.subject.meshAcute Disease-
dc.subject.meshAdolescent-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshChild-
dc.subject.meshChild, Preschool-
dc.subject.meshChromosomal Instability-
dc.subject.meshCpG Islands-
dc.subject.meshDNA Methylation-
dc.subject.meshFanconi Anemia Complementation Group F Protein-
dc.subject.meshFanconi Anemia Complementation Group Proteins-
dc.subject.meshGenes, Neoplasm-
dc.subject.meshHumans-
dc.subject.meshInfant-
dc.subject.meshLeukemia-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshNuclear Proteins-
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma-
dc.titleNo evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Paediatric Haematology and Oncology, Central Manchester and Manchester Children's University Hospitals NHS Trust, Manchester, UK. stefan.meyer-2@manchester.ac.uken
dc.identifier.journalBritish Journal of Haematologyen

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