Polar growth in the infectious hyphae of the phytopathogen ustilago maydis depends on a virulence-specific cyclin

2.50
Hdl Handle:
http://hdl.handle.net/10541/72846
Title:
Polar growth in the infectious hyphae of the phytopathogen ustilago maydis depends on a virulence-specific cyclin
Authors:
Flor-Parra, Ignacio; Castillo-Lluva, Sonia; Pérez-Simón, José A
Abstract:
The maize smut fungus Ustilago maydis switches from yeast to hyphal growth to infect maize (Zea mays) plants. This switching is promoted by mating of compatible cells and seems to be required for plant penetration. Although many genes distinctively expressed during this dimorphic switch have been identified and shown to be essential for the infection process, none seems to be explicitly required for polar growth control. Here, we report the characterization of pcl12, encoding a cyclin that interacts specifically with Cdk5, an essential cyclin-dependent kinase with regulatory roles in morphogenesis in U. maydis. Pcl12 fulfills the requirements to be a virulence-specific regulator of polar growth in U. maydis. First, pcl12 expression is induced during the pathogenic development. Secondly, Pcl12 is sufficient to induce hyperpolarized growth in U. maydis cells, as haploid cells overexpressing pcl12 in axenic conditions produce filaments that were morphologically indistinguishable from those produced during the infection process. Finally, cells defective in pcl12 showed impaired polar growth during the formation of the b-dependent filament, the induction of the conjugation tubes, or the formation of a promycelium in spore germination. However, in spite of this pivotal role during morphogenesis, pcl12 mutants were virulent. We discuss the implications of these results for the role of polar growth during the infection process.
Affiliation:
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Citation:
Polar growth in the infectious hyphae of the phytopathogen ustilago maydis depends on a virulence-specific cyclin. 2007, 19 (10):3280-96 Plant Cell
Journal:
The Plant Cell
Issue Date:
Oct-2007
URI:
http://hdl.handle.net/10541/72846
DOI:
10.1105/tpc.107.052738
PubMed ID:
17921314
Type:
Article
Language:
en
ISSN:
1040-4651
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFlor-Parra, Ignacio-
dc.contributor.authorCastillo-Lluva, Sonia-
dc.contributor.authorPérez-Simón, José A-
dc.date.accessioned2009-07-07T16:48:51Z-
dc.date.available2009-07-07T16:48:51Z-
dc.date.issued2007-10-
dc.identifier.citationPolar growth in the infectious hyphae of the phytopathogen ustilago maydis depends on a virulence-specific cyclin. 2007, 19 (10):3280-96 Plant Cellen
dc.identifier.issn1040-4651-
dc.identifier.pmid17921314-
dc.identifier.doi10.1105/tpc.107.052738-
dc.identifier.urihttp://hdl.handle.net/10541/72846-
dc.description.abstractThe maize smut fungus Ustilago maydis switches from yeast to hyphal growth to infect maize (Zea mays) plants. This switching is promoted by mating of compatible cells and seems to be required for plant penetration. Although many genes distinctively expressed during this dimorphic switch have been identified and shown to be essential for the infection process, none seems to be explicitly required for polar growth control. Here, we report the characterization of pcl12, encoding a cyclin that interacts specifically with Cdk5, an essential cyclin-dependent kinase with regulatory roles in morphogenesis in U. maydis. Pcl12 fulfills the requirements to be a virulence-specific regulator of polar growth in U. maydis. First, pcl12 expression is induced during the pathogenic development. Secondly, Pcl12 is sufficient to induce hyperpolarized growth in U. maydis cells, as haploid cells overexpressing pcl12 in axenic conditions produce filaments that were morphologically indistinguishable from those produced during the infection process. Finally, cells defective in pcl12 showed impaired polar growth during the formation of the b-dependent filament, the induction of the conjugation tubes, or the formation of a promycelium in spore germination. However, in spite of this pivotal role during morphogenesis, pcl12 mutants were virulent. We discuss the implications of these results for the role of polar growth during the infection process.en
dc.language.isoenen
dc.subject.meshCyclin-Dependent Kinase 5-
dc.subject.meshCyclins-
dc.subject.meshFungal Proteins-
dc.subject.meshGenome, Fungal-
dc.subject.meshHyphae-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutation-
dc.subject.meshProtein Binding-
dc.subject.meshUstilago-
dc.subject.meshVirulence-
dc.subject.meshZea mays-
dc.titlePolar growth in the infectious hyphae of the phytopathogen ustilago maydis depends on a virulence-specific cyclinen
dc.typeArticleen
dc.contributor.departmentDepartamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, 28049 Madrid, Spain.en
dc.identifier.journalThe Plant Cellen

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