Sodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72692
Title:
Sodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells.
Authors:
Katerinaki, Efthymia; Haycock, John W; Lalla, Ravindra; Carlson, Kenneth E; Yang, Yifan; Hill, Rebecca P; Lorigan, Paul C ( 0000-0002-8875-2164 ) ; MacNeil, Sheila
Abstract:
We have previously shown that tumour necrosis factor-alpha (TNF-alpha) upregulates human melanoma cell integrin expression, migration and invasion in vitro. The aim of this study was to investigate the effect of the non-steroidal anti-inflammatory agent sodium salicylate on TNF-alpha-induced activation of the transcription factor nuclear factor-kappaB (NF-kappaB) and upregulation of intercellular adhesion molecule-1 (ICAM-1), and TNF-alpha-stimulated cell migration and invasion through fibronectin. HBL human melanoma cells were pre-incubated with sodium salicylate prior to stimulation with TNF-alpha for 24 h. NF-kappaB activation was measured using an assay that detects changes in the expression of a luciferase reporter gene under the direct control of NF-kappaB transcriptional activity. The effect of sodium salicylate and TNF-alpha on HBL cell invasion over 20 h and migration over 24 h was studied using fibronectin invasion and 'scratch wound' migration models in vitro, as described previously. Sodium salicylate inhibited TNF-alpha-stimulated NF-kappaB activation in melanoma cells in a concentration-dependent manner, and this was achieved with pre-incubation times as short as 15 min. TNF-alpha-stimulated ICAM-1 expression in HBL cells was also downregulated by sodium salicylate, although in a manner inversely related to the concentration of this agent. In functional assays, TNF-alpha stimulated migration and invasion, and sodium salicylate significantly reduced the extent of melanoma invasion and migration in both the presence and absence of TNF-alpha. In conclusion, sodium salicylate effectively inhibited TNF-alpha-induced upregulation of NF-kappaB, ICAM-1 expression, in-vitro migration and invasion in human melanoma cells, indicating that non-steroidal anti-inflammatory drugs may be a useful therapeutic approach to oppose inflammation-induced melanoma invasion and metastasis in vivo.
Affiliation:
University of Sheffield, Section of Human Metabolism, Division of Clinical Sciences, Northern General Hospital, Sheffield, UK.
Citation:
Sodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells. 2006, 16 (1):11-22 Melanoma Res.
Journal:
Melanoma Research
Issue Date:
Feb-2006
URI:
http://hdl.handle.net/10541/72692
DOI:
10.1097/01.cmr.0000195698.58013.53
PubMed ID:
16432451
Type:
Article
Language:
en
ISSN:
0960-8931
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKaterinaki, Efthymia-
dc.contributor.authorHaycock, John W-
dc.contributor.authorLalla, Ravindra-
dc.contributor.authorCarlson, Kenneth E-
dc.contributor.authorYang, Yifan-
dc.contributor.authorHill, Rebecca P-
dc.contributor.authorLorigan, Paul C-
dc.contributor.authorMacNeil, Sheila-
dc.date.accessioned2009-07-07T11:05:37Z-
dc.date.available2009-07-07T11:05:37Z-
dc.date.issued2006-02-
dc.identifier.citationSodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells. 2006, 16 (1):11-22 Melanoma Res.en
dc.identifier.issn0960-8931-
dc.identifier.pmid16432451-
dc.identifier.doi10.1097/01.cmr.0000195698.58013.53-
dc.identifier.urihttp://hdl.handle.net/10541/72692-
dc.description.abstractWe have previously shown that tumour necrosis factor-alpha (TNF-alpha) upregulates human melanoma cell integrin expression, migration and invasion in vitro. The aim of this study was to investigate the effect of the non-steroidal anti-inflammatory agent sodium salicylate on TNF-alpha-induced activation of the transcription factor nuclear factor-kappaB (NF-kappaB) and upregulation of intercellular adhesion molecule-1 (ICAM-1), and TNF-alpha-stimulated cell migration and invasion through fibronectin. HBL human melanoma cells were pre-incubated with sodium salicylate prior to stimulation with TNF-alpha for 24 h. NF-kappaB activation was measured using an assay that detects changes in the expression of a luciferase reporter gene under the direct control of NF-kappaB transcriptional activity. The effect of sodium salicylate and TNF-alpha on HBL cell invasion over 20 h and migration over 24 h was studied using fibronectin invasion and 'scratch wound' migration models in vitro, as described previously. Sodium salicylate inhibited TNF-alpha-stimulated NF-kappaB activation in melanoma cells in a concentration-dependent manner, and this was achieved with pre-incubation times as short as 15 min. TNF-alpha-stimulated ICAM-1 expression in HBL cells was also downregulated by sodium salicylate, although in a manner inversely related to the concentration of this agent. In functional assays, TNF-alpha stimulated migration and invasion, and sodium salicylate significantly reduced the extent of melanoma invasion and migration in both the presence and absence of TNF-alpha. In conclusion, sodium salicylate effectively inhibited TNF-alpha-induced upregulation of NF-kappaB, ICAM-1 expression, in-vitro migration and invasion in human melanoma cells, indicating that non-steroidal anti-inflammatory drugs may be a useful therapeutic approach to oppose inflammation-induced melanoma invasion and metastasis in vivo.en
dc.language.isoenen
dc.subjectCancer Invasivenessen
dc.subjectSkin Canceren
dc.subjectTumour Cellsen
dc.subjectTumour Necrosisen
dc.subject.meshCell Movement-
dc.subject.meshFibronectins-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHumans-
dc.subject.meshIntercellular Adhesion Molecule-1-
dc.subject.meshLuciferases-
dc.subject.meshMelanoma-
dc.subject.meshNF-kappa B-
dc.subject.meshNeoplasm Invasiveness-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshSkin Neoplasms-
dc.subject.meshSodium Salicylate-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.subject.meshUp-Regulation-
dc.titleSodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells.en
dc.typeArticleen
dc.contributor.departmentUniversity of Sheffield, Section of Human Metabolism, Division of Clinical Sciences, Northern General Hospital, Sheffield, UK.en
dc.identifier.journalMelanoma Researchen
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