5.00
Hdl Handle:
http://hdl.handle.net/10541/72682
Title:
Microfluidic reactor for the radiosynthesis of PET radiotracers.
Authors:
Gillies, James M; Prenant, C; Chimon, G N; Smethurst, G J; Perrie, W; Hamblett, Ian; Dekker, Bronwen A; Zweit, Jamal
Abstract:
Here we show the first application of a microfabricated reaction system to PET radiochemistry, we term "microfluidic PET". The short half-life of the positron emitting isotopes and the trace chemical quantities used in radiolabelling make PET radiochemistry amenable to miniaturisation. Microfluidic technologies are capable of controlling and transferring tiny quantities of liquids which allow chemical and biochemical assays to be integrated and carried out on a small scale. Such technologies provide distinct advantages over current methods of PET radiochemical synthesis. To demonstrate "proof of principle" we have investigated the radiohalogenation of small and large molecular weight molecules using the microfluidic device. These reactions involved the direct radioiodination of the apoptosis marker Annexin V using iodine-124, the indirect radioiodination of the anti-cancer drug doxorubicin from a tin-butyl precursor and the radiosynthesis of 2-[(18)F]FDG from a mannose triflate precursor and fluorine-18 and hence provide a test bed for microfluidic reactions. We demonstrate the rapid radioiodination of the protein Annexin V (40% radiochemical yield within 1 min) and the rapid radiofluorination of 2-[(18)F]FDG (60% radiochemical yield within 4s) using a polymer microreactor chip. Chromatographic analysis showed that the labelling efficiency of the unoptimised microfluidic chip is comparable to conventional PET radiolabelling reactions.
Affiliation:
Cancer Research-UK/UMIST Radiochemical Targeting and Imaging Group, Paterson Institute for Cancer Research, Manchester M20 4BX, UK. jgillies@picr.man.ac.uk
Citation:
Microfluidic reactor for the radiosynthesis of PET radiotracers. 2006, 64 (3):325-32 Appl Radiat Isot
Journal:
Applied Radiation and Isotopes
Issue Date:
Mar-2006
URI:
http://hdl.handle.net/10541/72682
DOI:
10.1016/j.apradiso.2005.08.007
PubMed ID:
16290944
Type:
Article
Language:
en
ISSN:
0969-8043
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGillies, James M-
dc.contributor.authorPrenant, C-
dc.contributor.authorChimon, G N-
dc.contributor.authorSmethurst, G J-
dc.contributor.authorPerrie, W-
dc.contributor.authorHamblett, Ian-
dc.contributor.authorDekker, Bronwen A-
dc.contributor.authorZweit, Jamal-
dc.date.accessioned2009-07-07T10:09:00Z-
dc.date.available2009-07-07T10:09:00Z-
dc.date.issued2006-03-
dc.identifier.citationMicrofluidic reactor for the radiosynthesis of PET radiotracers. 2006, 64 (3):325-32 Appl Radiat Isoten
dc.identifier.issn0969-8043-
dc.identifier.pmid16290944-
dc.identifier.doi10.1016/j.apradiso.2005.08.007-
dc.identifier.urihttp://hdl.handle.net/10541/72682-
dc.description.abstractHere we show the first application of a microfabricated reaction system to PET radiochemistry, we term "microfluidic PET". The short half-life of the positron emitting isotopes and the trace chemical quantities used in radiolabelling make PET radiochemistry amenable to miniaturisation. Microfluidic technologies are capable of controlling and transferring tiny quantities of liquids which allow chemical and biochemical assays to be integrated and carried out on a small scale. Such technologies provide distinct advantages over current methods of PET radiochemical synthesis. To demonstrate "proof of principle" we have investigated the radiohalogenation of small and large molecular weight molecules using the microfluidic device. These reactions involved the direct radioiodination of the apoptosis marker Annexin V using iodine-124, the indirect radioiodination of the anti-cancer drug doxorubicin from a tin-butyl precursor and the radiosynthesis of 2-[(18)F]FDG from a mannose triflate precursor and fluorine-18 and hence provide a test bed for microfluidic reactions. We demonstrate the rapid radioiodination of the protein Annexin V (40% radiochemical yield within 1 min) and the rapid radiofluorination of 2-[(18)F]FDG (60% radiochemical yield within 4s) using a polymer microreactor chip. Chromatographic analysis showed that the labelling efficiency of the unoptimised microfluidic chip is comparable to conventional PET radiolabelling reactions.en
dc.language.isoenen
dc.subject.meshBioreactors-
dc.subject.meshEquipment Design-
dc.subject.meshEquipment Failure Analysis-
dc.subject.meshFeasibility Studies-
dc.subject.meshFluorodeoxyglucose F18-
dc.subject.meshIsotope Labeling-
dc.subject.meshMicrofluidic Analytical Techniques-
dc.subject.meshPositron-Emission Tomography-
dc.subject.meshRadiopharmaceuticals-
dc.titleMicrofluidic reactor for the radiosynthesis of PET radiotracers.en
dc.typeArticleen
dc.contributor.departmentCancer Research-UK/UMIST Radiochemical Targeting and Imaging Group, Paterson Institute for Cancer Research, Manchester M20 4BX, UK. jgillies@picr.man.ac.uken
dc.identifier.journalApplied Radiation and Isotopesen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.