Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields.
Authors
Sinclair, JohnWeeks, Mark E
Butt, Amna
Worthington, Jessica L
Akpan, Akunna
Jones, Nic
Waterfield, Michael D
Allan, Donald
Timms, John F
Affiliation
Ludwig Institute for Cancer Research, Department of Biochemistry and Molecular Biology University College London, UK.Issue Date
2006-09
Metadata
Show full item recordAbstract
There is considerable public concern regarding the health effects of exposure to low-frequency electromagnetic fields. In addition, the association between exposure and disease incidence or the possible biological effects of exposure are unclear. Using 2D-DIGE and MS in a blind study, we have investigated the effects of static and oscillating extremely low-frequency electromagnetic fields (ELF EMFs) on the proteomes of wild type Schizosaccharomyces pombe and a Sty1p deletion mutant which displays increased sensitivity to a variety of cellular stresses. Whilst this study identifies a number of protein isoforms that display significant differential expression across experimental conditions, there was no correlation between their patterns of expression and the ELF EMF exposure regimen. We conclude that there are no significant effects of either static or oscillating EMF on the yeast proteome at the sensitivity afforded by 2D-DIGE. We hypothesise that the proteins identified must be sensitive to subtle changes in culture and/or handling conditions, and that the identification of these proteins in other proteomic studies should be treated with some caution when the results of such studies are interpreted in a biological context.Citation
Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields. 2006, 6 (17):4755-64 ProteomicsJournal
ProteomicsDOI
10.1002/pmic.200500861PubMed ID
16897687Type
ArticleLanguage
enISSN
1615-9853ae974a485f413a2113503eed53cd6c53
10.1002/pmic.200500861
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