Sensitivity and specificity of immunohistochemical markers used in the diagnosis of epithelioid mesothelioma: a detailed systematic analysis using published data.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72633
Title:
Sensitivity and specificity of immunohistochemical markers used in the diagnosis of epithelioid mesothelioma: a detailed systematic analysis using published data.
Authors:
King, J E; Thatcher, Nick; Pickering, C A C; Hasleton, Philip S
Abstract:
AIMS: Immunohistochemistry is frequently employed to aid the distinction between mesothelioma and pulmonary adenocarcinoma metastatic to the pleura, but there is uncertainty as to which antibodies are most useful. We analysed published data in order to establish sensitivity and specificity of antibodies used to distinguish between these tumours with a view to defining the most appropriate immunohistochemical panel to use when faced with this diagnostic problem. METHODS AND RESULTS: A systematic analysis of the results of 88 published papers comparing immunohistochemical staining of a panel of antibodies in mesothelioma with epithelioid areas, and pulmonary adenocarcinoma metastatic to the pleura. Results for a total of 15 antibodies were analysed and expressed in terms of sensitivity and specificity. The most sensitive antibodies for identifying pulmonary adenocarcinoma were MOC-31 and BG8 (both 93%), whilst the most specific were monoclonal CEA (97%) and TTF-1 (100%). The most sensitive antibodies to identify epithelioid mesothelioma were CK5/6 (83%) and HBME-1 (85%). The most specific antibodies were CK5/6 (85%) and WT1 (96%). CONCLUSIONS: No single antibody is able to differentiate reliably between these two tumours. The use of a small panel of antibodies with a high combined sensitivity and specificity is recommended.
Affiliation:
South Manchester University Hospitals NHS Trust, Wythenshawe Hospital and Christie Hospital NHS Trust, Manchester, UK. julietk@talk21.com
Citation:
Sensitivity and specificity of immunohistochemical markers used in the diagnosis of epithelioid mesothelioma: a detailed systematic analysis using published data. 2006, 48 (3):223-32 Histopathology
Journal:
Histopathology
Issue Date:
Feb-2006
URI:
http://hdl.handle.net/10541/72633
DOI:
10.1111/j.1365-2559.2005.02331.x
PubMed ID:
16430468
Type:
Article
Language:
en
ISSN:
0309-0167
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKing, J E-
dc.contributor.authorThatcher, Nick-
dc.contributor.authorPickering, C A C-
dc.contributor.authorHasleton, Philip S-
dc.date.accessioned2009-07-06T14:55:28Z-
dc.date.available2009-07-06T14:55:28Z-
dc.date.issued2006-02-
dc.identifier.citationSensitivity and specificity of immunohistochemical markers used in the diagnosis of epithelioid mesothelioma: a detailed systematic analysis using published data. 2006, 48 (3):223-32 Histopathologyen
dc.identifier.issn0309-0167-
dc.identifier.pmid16430468-
dc.identifier.doi10.1111/j.1365-2559.2005.02331.x-
dc.identifier.urihttp://hdl.handle.net/10541/72633-
dc.description.abstractAIMS: Immunohistochemistry is frequently employed to aid the distinction between mesothelioma and pulmonary adenocarcinoma metastatic to the pleura, but there is uncertainty as to which antibodies are most useful. We analysed published data in order to establish sensitivity and specificity of antibodies used to distinguish between these tumours with a view to defining the most appropriate immunohistochemical panel to use when faced with this diagnostic problem. METHODS AND RESULTS: A systematic analysis of the results of 88 published papers comparing immunohistochemical staining of a panel of antibodies in mesothelioma with epithelioid areas, and pulmonary adenocarcinoma metastatic to the pleura. Results for a total of 15 antibodies were analysed and expressed in terms of sensitivity and specificity. The most sensitive antibodies for identifying pulmonary adenocarcinoma were MOC-31 and BG8 (both 93%), whilst the most specific were monoclonal CEA (97%) and TTF-1 (100%). The most sensitive antibodies to identify epithelioid mesothelioma were CK5/6 (83%) and HBME-1 (85%). The most specific antibodies were CK5/6 (85%) and WT1 (96%). CONCLUSIONS: No single antibody is able to differentiate reliably between these two tumours. The use of a small panel of antibodies with a high combined sensitivity and specificity is recommended.en
dc.language.isoenen
dc.subjectLung Canceren
dc.subjectPleural Canceren
dc.subjectTumour Markersen
dc.subjectCancer Antigensen
dc.subject.meshAdenocarcinoma-
dc.subject.meshAntibodies, Neoplasm-
dc.subject.meshAntigens, CD15-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshCadherins-
dc.subject.meshCalcium-Binding Protein, Vitamin D-Dependent-
dc.subject.meshCarcinoembryonic Antigen-
dc.subject.meshDiagnosis, Differential-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshLung Neoplasms-
dc.subject.meshMesothelioma-
dc.subject.meshPleural Neoplasms-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshThrombomodulin-
dc.subject.meshTumor Markers, Biological-
dc.subject.meshVimentin-
dc.titleSensitivity and specificity of immunohistochemical markers used in the diagnosis of epithelioid mesothelioma: a detailed systematic analysis using published data.en
dc.typeArticleen
dc.contributor.departmentSouth Manchester University Hospitals NHS Trust, Wythenshawe Hospital and Christie Hospital NHS Trust, Manchester, UK. julietk@talk21.comen
dc.identifier.journalHistopathologyen
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